| Rank | Heat | Lane | Nation | Swimmers | Time | Notes |
| 1 | 4 | 1 | | Matt Grevers
Cody Miller
Tim Phillips
Ryan Lochte | 3:31.06 | Q |
| 2 | 3 | 6 | | Mitch Larkin
Jake Packard
David Morgan
Kyle Chalmers | 3:31.86 | Q |
| 3 | 2 | 7 | | Camille Lacourt
Giacomo Perez-Dortona
Mehdy Metella
Fabien Gilot | 3:32.51 | Q |
| 4 | 4 | 4 | | Ryosuke Irie
Yasuhiro Koseki
Takuro Fujii
Shinri Shioura | 3:32.82 | Q |
| 5 | 1 | 5 | | Jan-Philip Glania
Hendrik Feldwehr
Steffen Deibler
Christoph Fildebrandt | 3:32.94 | Q |
| 6 | 2 | 6 | | Chris Walker-Hebborn
Ross Murdoch
Adam Barrett
Ben Proud | 3:33.37 | Q |
| 7 | 4 | 3 | | Radosław Kawęcki
Marcin Stolarski
Paweł Korzeniowski
Konrad Czerniak | 3:33.50 | Q, NR |
| 8 | 4 | 8 | | Grigory Tarasevich
Ilya Khomenko
Daniil Pakhomov
Alexandr Sukhorukov | 3:34.02 | Q |
| 9 | 3 | 3 | | Simone Sabbioni
Andrea Toniato
Matteo Rivolta
Marco Orsi | 3:34.59 | |
| 10 | 3 | 4 | | Guilherme Guido
Felipe Lima
Arthur Mendes
Marcelo Chierighini | 3:34.73 | |
| 11 | 4 | 0 | | Xu Jiayu
Li Xiang
Li Zhuhao
Lin Yongqing | 3:35.21 | |
| 12 | 2 | 1 | | Danas Rapšys
Giedrius Titenis
Tadas Duškinas
Mindaugas Sadauskas | 3:35.30 | NR |
| 13 | 2 | 2 | | Russell Wood
Richard Funk
Santo Condorelli
Yuri Kisil | 3:35.72 | |
| 14 | 3 | 1 | | Apostolos Christou
Panagiotis Samilidis
Andreas Vazaios
Kristian Golomeev | 3:37.86 | |
| 15 | 2 | 5 | | Pavel Sankovich
Ilya Shymanovich
Yauhen Tsurkin
Artsiom Machekin | 3:38.23 | |
| 16 | 3 | 2 | | Corey Main
Glenn Snyders
Bradlee Ashby
Matthew Stanley | 3:38.69 | |
| 17 | 1 | 4 | | Nils Liess
Yannick Käser
Nico van Duijn
Alexandre Haldemann | 3:38.94 | NR |
| 18 | 1 | 3 | | Doruk Tekin
Demir Atasoy
Kaan Türker Ayar
Kemal Arda Gürdal | 3:40.46 | NR |
| 19 | 2 | 0 | | Quah Zheng Wen
Khoo Chien Yin Lionel
Joseph Schooling
Yeo Kai Quan | 3:40.93 | |
| 20 | 3 | 7 | | Omar Pinzón
Jorge Murillo
Esnaider Reales
Mateo de Angulo | 3:41.44 | |
| 21 | 4 | 6 | | Mohamed Khaled
Youssef El-Kamash
Omar Eissa
Marwan El-Kamash | 3:41.97 | NR |
| 22 | 2 | 4 | | Daniil Bukin
Vladislav Mustafin
Aleksey Derlyugov
Daniil Tulupov | 3:42.33 | |
| 23 | 4 | 9 | | Ralf Tribuntsov
Martin Allikvee
Martin Liivamägi
Martti Aljand | 3:43.15 | |
| 24 | 3 | 5 | | Roman Dmytrijev
Petr Bartůněk
Jan Šefl
Martin Verner | 3:44.21 | |
| 25 | 2 | 8 | | Raphaël Stacchiotti
Laurent Carnol
Julien Henx
Pit Brandenburger | 3:44.22 | |
| 26 | 3 | 9 | | Janis Šaltans
Daniils Bobrovs
Nikolajs Maskalenko
Uvis Kalniņš | 3:45.58 | |
| 27 | 3 | 8 | | Yousef Al-Askari
Ahmad Al-Bader
Abbas Qali
Mohammad Madwa | 3:50.75 | NR |
| 2 | 3 | | | | DNS |
| 3 | 0 | | | | DNS |
| 4 | 5 | | | | DNS |
| 4 | 2 | | Carlos Omaña (DSQsort|9:99.99|FinalThe final was held at 19:07.
| Rank | Lane | Nation | Swimmers | Time | Notes | | 4 | | Ryan Murphy
Kevin Cordes
Tom Shields
Nathan Adrian | 3:29.93 | | | 5 | | Mitch Larkin
Jake Packard
Jayden Hadler
Cameron McEvoy | 3:30.08 | | | 3 | | Camille Lacourt
Giacomo Perez-Dortona
Mehdy Metella
Fabien Gilot | 3:30.50 | | | 4 | 7 | | Chris Walker-Hebborn
Adam Peaty
James Guy
Ben Proud | 3:30.67 | NR | | 5 | 8 | | Evgeny Rylov
Kirill Prigoda
Daniil Pakhomov
Vladimir Morozov | 3:30.90 | | | 6 | 6 | | Ryosuke Irie
Yasuhiro Koseki
Takuro Fujii
Shinri Shioura | 3:31.10 | | | 7 | 2 | | Jan-Philip Glania
Hendrik Feldwehr
Steffen Deibler
Christoph Fildebrandt | 3:32.16 | | | 8 | 1 | | Radosław Kawęcki
Marcin Stolarski
Paweł Korzeniowski
Konrad Czerniak | 3:34.34 | |
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