High-altitude adaptation in humans
High-altitude adaptation in humans is an instance of evolutionary modification in certain human populations, including those of Tibet in Asia, the Andes of the Americas, and Ethiopia in Africa, who have acquired the ability to survive at extremely high altitudes. This adaptation means irreversible, long-term physiological responses to high-altitude environments, associated with heritable behavioural and genetic changes. While the rest of the human population would suffer serious health consequences, the indigenous inhabitants of these regions thrive well in the highest parts of the world. These people have undergone extensive physiological and genetic changes, particularly in the regulatory systems of oxygen respiration and blood circulation, when compared to the general lowland population.
Around 140 million people, just under 2% of the world's human population, live permanently at altitudes above in South America, East Africa, and South Asia. These populations have done so for millennia without apparent complications. This special adaptation is now recognised as an example of natural selection in action. The adaptation of the Tibetans is the fastest known example of human evolution, as it is estimated to have occurred any time around 1,000 B.C.E. to 7,000 B.C.E.
Origin and basis
are naturally adapted to lowland environment where oxygen is abundant. When people from the general lowlands go to altitudes above they experience altitude sickness, which is a type of hypoxia, a clinical syndrome of severe lack of oxygen. Some people get the illness even at above 1,500 metres. Complications include fatigue, dizziness, breathlessness, headaches, insomnia, malaise, nausea, vomiting, body pain, loss of appetite, ear-ringing, blistering and purpling of the hands and feet, and dilated veins.The sickness is compounded by related symptoms such as cerebral oedema and pulmonary oedema. For several days, they breathe excessively and burn extra energy even when the body is relaxed. The heart rate then gradually decreases. Hypoxia, in fact, is one of the principal causes of death among mountaineers. In women, pregnancy can be severely affected, such as development of high blood pressure, called preeclampsia, which causes premature labour, low birth weight of babies, and often complicated with profuse bleeding, seizures, and death of the mother.
More than 140 million people worldwide are estimated to live at an elevation higher than above sea level, of which 13 million are in Ethiopia, 1.7 million in Tibet, 35 million in the South American Andes, and 0.3 million in Colorado Rocky Mountains. Certain natives of Tibet, Ethiopia, and the Andes have been living at these high altitudes for generations and are protected from hypoxia as a consequence of genetic adaptation. It is estimated that at, every lungful of air only has 60% of the oxygen molecules that people at sea level have. Highlanders are thus constantly exposed to a low oxygen environment, yet they live without any debilitating problems. One of the best documented effects of high altitude is a progressive reduction in birth weight. It has been known that women of long-resident high-altitude population are not affected. These women are known to give birth to heavier-weight infants than women of lowland inhabitants. This is particularly true among Tibetan babies, whose average birth weight is 294–650 g heavier than the surrounding Chinese population; and their blood-oxygen level is considerably higher.
The first scientific investigations of high-altitude adaptation was done by A. Roberto Frisancho of the University of Michigan in the late 1960s among the Quechua people of Peru. Paul T. Baker, Penn State University, also conducted a considerable amount of research into human adaptation to high altitudes, and mentored students who continued this research. One of these students went on to conduct research on high altitude adaptation among the Tibetans in the early 1980s through to today, anthropologist Cynthia Beall of the Case Western Reserve University.
Physiological basis
Tibetans
Scientists started to notice the extraordinary physical performance of Tibetans since the beginning of Himalayan climbing era in the early 20th century. The hypothesis of a possible evolutionary genetic adaptation makes sense. The Tibetan plateau has an average elevation of above sea level, and covering more than 2.5 million km2, it is the highest and largest plateau in the world. In 1990, it was estimated that 4,594,188 Tibetans live on the plateau, with 53% living at an altitude over. Fairly large numbers live at an altitude exceeding in the Chantong-Qingnan area. Where the Tibetan highlanders live, the oxygen level is only about 60% of that at sea level. The Tibetans, who have been living in this region for 3,000 years, do not exhibit the elevated haemoglobin concentrations to cope with oxygen deficiency as observed in other populations who have moved temporarily or permanently at high altitudes. Instead, the Tibetans inhale more air with each breath and breathe more rapidly than either sea-level populations or Andeans. Tibetans have better oxygenation at birth, enlarged lung volumes throughout life, and a higher capacity for exercise. They show a sustained increase in cerebral blood flow, lower haemoglobin concentration, and less susceptibility to chronic mountain sickness than other populations, due to their longer history of high-altitude habitation.General people can develop short-term tolerance with careful physical preparation and systematic monitoring of movements, but the biological changes are quite temporary and reversible when they return to lowlands. Moreover, unlike lowland people who only experience increased breathing for a few days after entering high altitudes, Tibetans retain this rapid breathing and elevated lung-capacity throughout their lifetime. This enables them to inhale larger amounts of air per unit of time to compensate for low oxygen levels. In addition, they have high levels of nitric oxide in their blood, when compared to lowlanders, and this probably helps their blood vessels dilate for enhanced blood circulation. Further, their haemoglobin level is not significantly different, from those of people living at low altitude. In this way they are able to evade both the effects of hypoxia and mountain sickness throughout life. Even when they climbed the highest summits like Mt. Everest, they showed regular oxygen uptake, greater ventilation, more brisk hypoxic ventilatory responses, larger lung volumes, greater diffusing capacities, constant body weight and a better quality of sleep, compared to people from the lowland.
Andeans
In contrast to the Tibetans, the Andean highlanders, who have been living at high altitudes for no more than 11,000 years, show different pattern of haemoglobin adaptation. Their haemoglobin concentration is higher compared to those of lowlander population, which also happens to lowlanders moving to high altitude. When they spend some weeks in the lowland their haemoglobin drops to average of other people. This shows only temporary and reversible acclimatisation. However, in contrast to lowland people, they do have increased oxygen level in their haemoglobin, that is, more oxygen per blood volume than other people. This confers an ability to carry more oxygen in each red blood cell, making a more effective transport of oxygen in their body, while their breathing is essentially at the same rate. This enables them to overcome hypoxia and normally reproduce without risk of death for the mother or baby. The Andean highlanders are known from the 16th-century missionaries that their reproduction had always been normal, without any effect in the giving birth or the risk for early pregnancy loss, which are common to hypoxic stress. They have developmentally acquired enlarged residual lung volume and its associated increase in alveolar area, which are supplemented with increased tissue thickness and moderate increase in red blood cells. Though the physical growth in body size is delayed, growth in lung volumes is accelerated. An incomplete adaptation such as elevated haemoglobin levels still leaves them at risk for mountain sickness with old age.Among the Quechua people of the Altiplano, there is a significant variation in NOS3, which is associated with higher levels of nitric oxide in high altitude. Nuñoa children of Quechua ancestry exhibit higher blood-oxygen content and lower heart rate than their counterpart school children of different ethnicity, who have an average of 89.9 blood-oxygen and 88–91 heart rate. High-altitude born and bred females of Quechua origins have comparatively enlarged lung volume for increased respiration.
Blood profile comparisons show that among the Andeans, Aymaran highlanders are better adapted to highlands than the Quechuas. Among the Bolivian Aymara people, the resting ventilation and hypoxic ventilatory response were quite low, in contrast to those of the Tibetans. The intrapopulation genetic variation was relatively less among the Aymara people. Moreover, when compared to Tibetans, the blood haemoglobin level at high altitudes among Aymarans is notably higher, with an average of 19.2 g/dl for males and 17.8 g/dl for females. Among the different native highlander populations, the underlying physiological responses to adaptation are quite different. For example, among four quantitative features, such as are resting ventilation, hypoxic ventilatory response, oxygen saturation, and haemoglobin concentration, the levels of variations are significantly different between the Tibetans and the Aymaras.
Ethiopians
The peoples of the Ethiopian highlands also live at extremely high altitudes, around to. Highland Ethiopians exhibit elevated haemoglobin levels, like Andeans and lowlander peoples at high altitudes, but do not exhibit the Andeans’ increase in oxygen content of haemoglobin. Among healthy individuals, the average haemoglobin concentrations are 15.9 and 15.0 g/dl for males and females respectively, and an average oxygen saturation of haemoglobin is 95.3%. Additionally, Ethiopian highlanders do not exhibit any significant change in blood circulation of the brain, which has been observed among the Peruvian highlanders. Yet, similar to the Andeans and Tibetans, the Ethiopian highlanders are immune to the extreme dangers posed by high-altitude environment, and their pattern of adaptation is definitely unique from that of other highland peoples.Genetic basis
The underlying molecular evolution of high-altitude adaptation has been explored and understood fairly recently. Depending on the geographical and environmental pressures, high-altitude adaptation involves different genetic patterns, some of whichhave evolved quite recently. For example, Tibetan adaptations became prevalent in the past 3,000 years, a rapid example of recent human evolution. At the turn of the 21st century, it was reported that the genetic make-up of the respiratory components of the Tibetan and the Ethiopian populations are significantly different.
Tibetans
Substantial evidence in Tibetan highlanders suggests that variation in haemoglobin and blood-oxygen levels are adaptive as Darwinian fitness. It has been documented that Tibetan women with a high likelihood of possessing one to two alleles for high blood-oxygen content had more surviving children; the higher the oxygen capacity, the lower the infant mortality. In 2010, for the first time, the genes responsible for the unique adaptive traits were identified following genome sequencing of 50 Tibetans and 40 Han Chinese from Beijing. Initially, the strongest signal of natural selection detected was a transcription factor involved in response to hypoxia, called endothelial Per-Arnt-Sim domain protein 1. It was found that one single-nucleotide polymorphism at EPAS1 shows a 78% frequency difference between Tibetan and mainland Chinese samples, representing the fastest genetic change observed in any human gene to date. Hence, Tibetan adaptation to high altitude becomes the fastest process of phenotypically observable evolution in humans, which is estimated to occur a few thousand years ago, when the Tibetans split up from the mainland Chinese population. The time of genetic divergence has been variously estimated as 2,750, 4,725, 8,000, or 9,000 years ago. Mutations in EPAS1, at higher frequency in Tibetans than their Han neighbours, correlate with decreased haemoglobin concentrations among the Tibetans, which is the hallmark of their adaptation to hypoxia. Simultaneously, two genes, egl nine homolog 1 and peroxisome proliferator-activated receptor alpha, were also identified to be positively selected in relation to decreased haemoglobin nature in the Tibetans.Similarly, the Sherpas, known for their Himalayan hardiness, exhibit similar patterns in the EPAS1 gene, which further fortifies that the gene is under selection for adaptation to the high-altitude life of Tibetans. A study in 2014 indicates that the mutant EPAS1 gene could have been inherited from archaic hominins, the Denisovans. EPAS1 and EGLN1 are definitely the major genes for unique adaptive traits when compared with those of the Chinese and Japanese. Comparative genome analysis in 2014 revealed that the Tibetans inherited an equal mixture of genomes from the Nepalese-Sherpas and Hans, and they acquired the adaptive genes from the sherpa-lineage. Further, the population split was estimated to occur around 20,000 to 40,000 years ago, a range of which support archaeological, mitochondria DNA and Y chromosome evidence for an initial colonisation of the Tibetan plateau around 30,000 years ago.
The genes function in concert with another gene named hypoxia inducible factors, which in turn is a principal regulator of red blood cell production in response to oxygen metabolism. The genes are associated not only with decreased haemoglobin levels, but also in regulating energy metabolism. EPAS1 is significantly associated with increased lactate concentration, and PPARA is correlated with decrease in the activity of fatty acid oxidation. EGLN1 codes for an enzyme, prolyl hydroxylase 2, involved in erythropoiesis. Among the Tibetans, mutation in EGLN1 results in mutant PHD2 and this mutation inhibits erythropoiesis. The mutation is estimated to occur about 8,000 years ago. Further, the Tibetans are enriched for genes in the disease class of human reproduction and biological process categories of response to DNA damage stimulus and DNA repair, which are related to the adaptive traits of high infant birth weight and darker skin tone and are most likely due to recent local adaptation.