HITS-CLIP


High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation is a genome-wide means of mapping protein-RNA binding sites or RNA modification sites in vivo. HITS-CLIP was originally used to generate genome-wide protein-RNA interaction maps for the neuron-specific RNA-binding protein and splicing factor NOVA1 and NOVA2; since then a number of other splicing factor maps have been generated, including those for PTB, RbFox2, SFRS1, hnRNP C, and even N6-Methyladenosine mRNA modifications.
HITS-CLIP of the RNA-binding protein Argonaute has been performed for the identification of microRNA targets by decoding microRNA-mRNA and protein-RNA interaction maps in mouse brain, and subsequently in Caenorhabditis elegans, embryonic stem cells and tissue culture cells.
As a novel modification of HITS-CLIP, m6A-CLIP was developed to precisely map N6-Methyladenosine locations in mRNA by UV-crosslinking m6A antibody to the target RNA. Recently, improved bioinformatics applied to Argonaute HITS-CLIP enables identification of binding sites with single nucleotide resolution.

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