Embryo transfer
Embryo transfer refers to a step in the process of assisted reproduction in which embryos are placed into the uterus of a female with the intent to establish a pregnancy. This technique, may be used in humans or in animals, in which situations the goals may vary.
Embryo transfer can be done at day two or day three, or later in the blastocyst stage, which was first performed in 1984.
Fresh versus frozen
Embryos can be either “fresh” from fertilized egg cells of the same menstrual cycle, or “frozen”, that is they have been generated in a preceding cycle and undergone embryo cryopreservation, and are thawed just prior to the transfer, which is then termed "frozen embryo transfer". The outcome from using cryopreserved embryos has uniformly been positive with no increase in birth defects or development abnormalities, also between fresh versus frozen eggs used for intracytoplasmic sperm injection. In fact, pregnancy rates are increased following FET, and perinatal outcomes are less affected, compared to embryo transfer in the same cycle as ovarian hyperstimulation was performed. The endometrium is believed to not be optimally prepared for implantation following ovarian hyperstimulation, and therefore frozen embryo transfer avails for a separate cycle to focus on optimizing the chances of successful implantation. Children born from vitrified blastocysts have significantly higher birthweight than those born from non-frozen blastocysts. When transferring a frozen-thawed oocyte, the chance of pregnancy is essentially the same whether it is transferred in a natural cycle or one with ovulation induction.There is probably little or no difference between FET and fresh embryo transfers in terms of live birth rate; however, FET may lower the risk of ovarian hyperstimulation syndrome while at the same time it may increase the risk of pregnancy complications.
Uterine preparation
In the human, the uterine lining needs to be appropriately prepared so that the embryo can implant. In a natural cycle the embryo transfer takes place in the luteal phase at a time where the lining is appropriately undeveloped in relation to the status of the present Luteinizing Hormone. In a stimulated or a cycle where a "frozen" embryo is transferred, the recipient woman could be given first estrogen preparations, then a combination of estrogen and progesterone so that the lining becomes receptive for the embryo. The time of receptivity is the implantation window. A scientific review in 2013 came to the conclusion that it is not possible to identify one method of endometrium preparation in frozen embryo transfer as being more effective than another.Limited evidence also supports removal of cervical mucus before transfer.
Timing
Embryo transfer can be performed after various durations of embryo culture, conferring different stages in embryogenesis. The main stages at which embryo transfer is performed are cleavage stage or the blastocyst stage.Embryos who reach the day 3 cell stage can be tested for chromosomal or specific genetic defects prior to possible transfer by preimplantation genetic diagnosis. Transferring at the blastocyst stage confers a significant increase in live birth rate per transfer, but also confers a decreased number of embryos available for transfer and embryo cryopreservation, so the cumulative clinical pregnancy rates are increased with cleavage stage transfer. It is uncertain whether there is any difference in live birth rate between transfer on day two or day three after fertilization.
Monozygotic twinning is not increased after blastocyst transfer compared with cleavage-stage embryo transfer.
There is a significantly higher odds of preterm birth and congenital anomalies among births having reached the blastocyst stage compared with cleavage stage. Because of increased female embryo mortality due to epigenetic modifications induced by extended culture, blastocyst transfer leads to more male births versus 2 or 3 day transfer.
Embryo selection
Laboratories have developed grading methods to judge oocyte and embryo quality. In order to optimise pregnancy rates, there is significant evidence that a morphological scoring system is the best strategy for the selection of embryos. Since 2009 where the first time-lapse microscopy system for IVF was approved for clinical use, morphokinetic scoring systems has shown to improve to pregnancy rates further. However, when all different types of time-lapse embryo imaging devices, with or without morphokinetic scoring systems, are compared against conventional embryo assessment for IVF, there is insufficient evidence of a difference in live-birth, pregnancy, stillbirth or miscarriage to choose between them. A small prospectively randomized study in 2016 reported poorer embryo quality and more staff time in an automated time-lapse embryo imaging device compared to conventional embryology. Active efforts to develop a more accurate embryo selection analysis based on Artificial Intelligence and Deep Learning are underway. Embryo Ranking Intelligent Classification Algorithm, is a clear example. This Deep Learning software substitutes manual classifications with a ranking system based on an individual embryo's predicted genetic status in a non-invasive fashion. Studies on this area are still pending and current feasibility studies support its potential.Procedure
The embryo transfer procedure starts by placing a speculum in the vagina to visualize the cervix, which is cleansed with saline solution or culture media. A soft transfer catheter is loaded with the embryos and handed to the clinician after confirmation of the patient's identity. The catheter is inserted through the cervical canal and advanced into the uterine cavity.There is good and consistent evidence of benefit in ultrasound guidance, that is, making an abdominal ultrasound to ensure correct placement, which is 1–2 cm from the uterine fundus. There is evidence of a significant increase in clinical pregnancy using ultrasound guidance compared with only "clinical touch". Anesthesia is generally not required. Single embryo transfers in particular require accuracy and precision in placement within the uterine cavity. The optimal target for embryo placement, known as the maximal implantation potential point, is identified using 3D/4D ultrasound. However, there is limited evidence that supports deposition of embryos in the midportion of the uterus.
After insertion of the catheter, the contents are expelled and the embryos are deposited. Limited evidence supports making trial transfers before performing the procedure with embryos. After expulsion, the duration that the catheter remains inside the uterus has no effect on pregnancy rates. Limited evidence suggests avoiding negative pressure from the catheter after expulsion. After withdrawal, the catheter is handed to the embryologist, who inspects it for retained embryos.
In the process of zygote intrafallopian transfer, eggs are removed from the woman, fertilised, and then placed in the woman's fallopian tubes rather than the uterus.
Procedure simulation
In 2015, the American Society for Reproductive Medicine developed a medical simulation of the Embryo Transfer procedure with Swiss company VirtaMed, designed for the education and training of clinicians. The virtual reality simulator, which includes real-time simulation of ultrasound guidance, was launched at the annual conference of the American Society for Reproductive Medicine.Embryo number
A major issue is how many embryos should be transferred. Placement of multiple embryos carries the risk of multiple pregnancy. In the past, physicians have often placed too many embryos in the hope to establish a pregnancy. However, the rise in multiple pregnancies has led to a reassessment of this approach. Professional societies and in many countries, the legislature, have issued guidelines or laws to curtail a practice of placing too many embryos in an attempt to reduce multiple pregnancies. The appropriate number of embryos to be transferred depends on the age of the woman, whether it is the first, second or third full IVF cycle attempt and whether there are top-quality embryos available. According to a guideline from The National Institute for Health and Care Excellence in 2013, the number of embryos transferred in a cycle should be chosen as in following table:| Age | Attempt No. | Embryos transferred |
| <37 years | 1st | 1 |
| <37 years | 2nd | 1 if top-quality |
| <37 years | 3rd | No more than 2 |
| 37–39 years | 1st & 2nd | 1 if top-quality |
| 37–39 years | 1st & 2nd | 2 if no top-quality |
| 37–39 years | 3rd | No more than 2 |
| 40–42 years | 2 |
e-SET
The technique of selecting only one embryo to transfer to the woman is called elective-single embryo transfer or, when embryos are at the blastocyst stage, it can also be called elective single blastocyst transfer . It significantly lowers the risk of multiple pregnancies, compared with e.g. Double Embryo Transfer or double blastocyst transfer, with a twinning rate of approximately 3.5% in sET compared with approximately 38% in DET, or 2% in eSBT compared with approximately 25% in 2BT. At the same time, pregnancy rates is not significantly less with eSBT than with 2BT. That is, the cumulative live birth rate associated with single fresh embryo transfer followed by a single frozen and thawed embryo transfer is comparable with that after one cycle of double fresh embryo transfer. Furthermore, SET has better outcomes in terms of mean gestational age at delivery, mode of delivery, birthweight, and risk of neonatal intensive care unit necessity than DET. e-SET of embryos at the cleavage stage reduces the likelihood of live birth by 38% and multiple birth by 94%. Evidence from randomized, controlled trials suggests that increasing the number of e-SET attempts results in a cumulative live birth rate similar to that of DET.The usage of single embryo transfer is highest in Sweden, but as low as 2.8% in the USA. Access to public funding for ART, availability of good cryopreservation facilities, effective education about the risks of multiple pregnancy, and legislation appear to be the most important factors for regional usage of single embryo transfer. Also, personal choice plays a significant role as many subfertile couples have a strong preference for twins.
Adjunctive procedures
It is uncertain whether the use of mechanical closure of the cervical canal following embryo transfer has any effect.Whether or not women remain laying down for a certain amount of time after embryo transfer may make little or no difference.
Using hyaluronic acid as an adherence medium for the embryo may increase live birth rates. There may be little or benefit in having a full bladder, removal of cervical mucus, or flushing of the endometrial or endocervical cavity at the time of embryo transfer. Adjunctive antibiotics in the form of amoxicillin plus clavulanic acid probably does not increase the clinical pregnancy rate compared with no antibiotics.
For frozen-thawed embryo transfer or transfer of embryo from egg donation, no previous ovarian hyperstimulation is required for the recipient before transfer, which can be performed in spontaneous ovulatory cycles. Still, various protocols exist for frozen-thawed embryo transfers as well, such as protocols with ovarian hyperstimulation, protocols in which the endometrium is artificially prepared by estrogen and/or progesterone. There is some evidence that in cycles where the endometrium is artificially prepared by estrogen or progesterone, it may be beneficial to administer an additional drug that suppresses hormone production by the ovaries such as continuous administration of a gonadotropin releasing hormone agonist. For egg donation, there is evidence of a lower pregnancy rate and a higher cycle cancellation rate when the progesterone supplementation in the recipient is commenced prior to oocyte retrieval from the donor, as compared to commenced day of oocyte retrieval or the day after.
Seminal fluid contains several proteins that interact with epithelial cells of the cervix and uterus, inducing active gestational immune tolerance. There are significantly improved outcomes when women are exposed to seminal plasma around the time of embryo transfer, with statistical significance for clinical pregnancy, but not for ongoing pregnancy or live birth rates with the limited data available.
Follow-up
Patients usually start progesterone medication after egg retrieval. While daily intramuscular injections of progesterone-in-oil have been the standard route of administration, PIO injections are not FDA-approved for use in pregnancy. A recent meta-analysis showed that the intravaginal route with an appropriate dose and dosing frequency is equivalent to daily intramuscular injections. In addition, a recent case-matched study comparing vaginal progesterone with PIO injections showed that live birth rates were nearly identical with both methods. A duration of progesterone administration of 11 days results in almost the same birth rates as longer durations.Patients are also given estrogen medication in some cases after the embryo transfer. Pregnancy testing is done typically two weeks after egg retrieval.
Third-party reproduction
It is not necessary that the embryo transfer be performed on the female who provided the eggs. Thus another female whose uterus is appropriately prepared can receive the embryo and become pregnant.Embryo transfer may be used where a woman who has eggs but no uterus and wants to have a biological baby; she would require the help of a gestational carrier or surrogate to carry the pregnancy. Also, a woman who has no eggs but a uterus may utilize egg donor IVF, in which case another woman would provide eggs for fertilization and the resulting embryos are placed into the uterus of the patient. Fertilization may be performed using the woman's partner's sperm or by using donor sperm. 'Spare' embryos which are created for another couple undergoing IVF treatment but which are then surplus to that couple's needs may also be transferred. Embryos may be specifically created by using eggs and sperm from donors and these can then be transferred into the uterus of another woman. A surrogate may carry a baby produced by embryo transfer for another couple, even though neither she nor the 'commissioning' couple is biologically related to the child. Third party reproduction is controversial and regulated in many countries. Persons entering gestational surrogacy arrangements must make sense of an entirely new type of relationship that does not fit any of the traditional scripts we use to categorize relations as kinship, friendship, romantic partnership or market relations. Surrogates have the experience of carrying a baby that they conceptualize as not of their own kin, while intended mothers have the experience of waiting through nine months of pregnancy and transitioning to motherhood from outside of the pregnant body. This can lead to new conceptualizations of body and self.
History
The first transfer of an embryo from one human to another resulting in pregnancy was reported in July 1983 and subsequently led to the announcement of the first human birth 3 February 1984. This procedure was performed at the Harbor UCLA Medical Center under the direction of Dr. John Buster and the University of California at Los Angeles School of Medicine.In the procedure, an embryo that was just beginning to develop was transferred from one woman in whom it had been conceived by artificial insemination to another woman who gave birth to the infant 38 weeks later. The sperm used in the artificial insemination came from the husband of the woman who bore the baby.
This scientific breakthrough established standards and became an agent of change for women suffering from the afflictions of infertility and for women who did not want to pass on genetic disorders to their children. Donor embryo transfer has given women a mechanism to become pregnant and give birth to a child that will contain their husband's genetic makeup. Although donor embryo transfer as practiced today has evolved from the original non-surgical method, it now accounts for approximately 5% of in vitro fertilization recorded births.
Prior to this, thousands of women who were infertile, had adoption as the only path to parenthood. This set the stage to allow open and candid discussion of embryo donation and transfer. This breakthrough has given way to the donation of human embryos as a common practice similar to other donations such as blood and major organ donations. At the time of this announcement the event was captured by major news carriers and fueled healthy debate and discussion on this practice which impacted the future of reproductive medicine by creating a platform for further advancements in woman's health.
This work established the technical foundation and legal-ethical framework surrounding the clinical use of human oocyte and embryo donation, a mainstream clinical practice, which has evolved over the past 25 years.
Effectiveness
A Cochrane systematic review updated in 2012 showed that blastocyst stage transfer is more effective than cleavage stage transfer in assisted reproductive technologies. It showed a small improvement in live birth rate per couple for blastocyst transfers. This would mean that for a typical rate of 31% in clinics that use early cleavage stage cycles, the rate would increase to 32% to 42% live births if clinics used blastocyst transfer.Embryo transfer in animals
Embryo transfer techniques allow top quality female livestock to have a greater influence on the genetic advancement of a herd or flock in much the same way that artificial insemination has allowed greater use of superior sires. ET also allows the continued use of animals such as competition mares to continue training and showing, while producing foals. The general epidemiological aspects of embryo transfer indicates that the transfer of embryos provides the opportunity to introduce genetic material into populations of livestock while greatly reducing the risk for transmission of infectious diseases. Recent developments in the sexing of embryos before transfer and implanting has great potential in the dairy and other livestock industries.Embryo transfer is also used in laboratory mice. For example, embryos of genetically modified strains that are difficult to breed or expensive to maintain may be stored frozen, and only thawed and implanted into a pseudopregnant dam when needed.
On February 19, 2020, the first pair of Cheetah cubs to be conceived through embryo transfer from a surrogate cheetah mother was born at Columbus Zoo in Ohio.