Thioesterase


Thioesterases are enzymes which belong to the esterase family. Esterases, in turn, are one type of the several hydrolases known.
Thioesterases exhibit Esterase activity specifically at a thiol group.
Thioesterases or thiolester hydrolases are identified as members of E.C.3.1.2.

Family

The thioesterase activity is performed by members of the acyl-CoA thioesterase family. The regulatory role of ACOT in fatty acid metabolism depends on their substrate specificity, tissue expression and subcellular localization. For example, deactivation of fatty acids at the ER may traffic fatty acids away from pathways associated with the ER membrane, such as glycerolipid biosynthesis. Two structurally different ACOT types lead to a similar enzymatic activity in vitro, dividing the family into type I and type II ACOTs.
Type I ACOTs contain the α/β-hydrolase domain, which is also present in many lipases and esterases.
Type II ACOTs have a characteristic structural motif called the ‘Hotdog fold’ domain.

Examples

, palmitoyl-CoA hydrolase, succinyl-CoA hydrolase, formyl-CoA hydrolase, acyl-CoA hydrolase are a few examples of this group of enzymes.
Ubiquitin thiolesterase is a well-known example, whose structure has been analyzed.
Humans genes which encode thioesterases include:
ACOT1, ACOT2, ACOT4, ACOT6, ACOT7, ACOT8, ACOT9, ACOT11, ACOT12, OLAH, PPT1, PPT2, THEM2, THEM4, THEM4P1, THEM5