SC 341 begins at the intersection of Georgetown Highway and Broadway Street in central Johnsonville. SC 341 heads west along East Broadway Street through the town's business district. At a railroad crossing, the road name changes to West Broadway and continues past additional businesses. After exiting the city limits, the road's name changes to Lake City Highway but passes some other small businesses, houses, and churches. Further from the city, more farmland begins to surround the roadway. At the community of Prospect Corner, the road name becomes Johnsonville Highway but the highway's surroundings is generally the same. Before entering Lake City, SC 341 passes in front of some commercial and industrial warehouses. Upon entering Lake City, SC 341 travels through it on Main Street, first through a residential neighborhood with an elementary school located along it. At Church Street, US 378 Bus. joins SC 341 on a concurrency through the city. Commercial businesses line both sides of the street upon the beginning of the concurrency. In the center of the city, the two routes intersect US 52. After passing Matthews Road west of the downtown area, the road enters another residential neighborhood but then heads into an area with more farmlands. Upon its intersection with US 378, US 378 Bus. ends and SC 341 has a short concurrency with US 378. Continuing northwest from US 378, SC 341 travels along Olanta Highway. At the community of Camp Branch, the highway intersects SC 403 at its southern terminus. SC 341 continues through mostly farmland and afterwards intersects SC 541 at its southern terminus. Shortly thereafter, the road enters the town of Olanta on Hampton Street. Within the town, the road passes in front of many houses, some small businesses, a library, and a school. In the center of town, SC 341 intersects US 301 then begins to curve to the north. The road heads through a mix of farmland and woods north of Olanta where it exits Florence County and enters Sumter County. After an intersection with SC 53, SC 341 begins to curve to the northwest and has an interchange with Interstate 95 at its exit 146. After entering Lee County, SC 341 reaches the community of South Lynchburg where it intersects SC 58 at its western terminus. Soon after, it enters the town of Lynchburg where in the center, it has an intersection with US 76. It continues northwest along Lynchburg Highway through farmland and intersects US 401 about northeast of Elliott. At the settlement of Wisacky, SC 341 reaches the northern terminus of SC 527. Now approaching the city of Bishopville, the highway passes in front of Lee Central High School and has an interchange with I-20 at exit 120. North of the Interstate, it passes the entrance to Lee Correctional Institution. Upon entering the city of Bishopville, it takes the name of East Church Street where it heads through a residential neighborhood then transitions to the business district. In the town center, SC 341 reaches Main Street which carries US 15 through the city. SC 341 and SC 34 form a concurrency with US 15 and head northeast through Bishopville. Outside the city limits, SC 341 breaks off the US 15/SC 34 concurrency and heads north-northwest through farmland and woods on Bethune Highway. The highway enters Kershaw County and soon afterwards heads into the town of Bethune. It travels through the town on Main Street where it intersects US 1 and SC 346. Outside of Bethune, it heads through the pine forests of the Sandhills region. After crossing the Little Lynches River, the road enters Lancaster County. It then enters the town of Kershaw on South Minor Street. At Marion Street, SC 341 turns to the west while SC 157 begins and travels east on Marion Street and SC 157 Truck / SC 341 Truck head north on North Minor Street. The highway heads into the center of town where SC 341 ends at Hampton Street which carries US 521 Bus./US 601 Bus. Marion Street continues west to intersect US 501 and US 601 at Matson Street.
Major junctions
Special route
SC 341 Truck is a truck route for trucks traveling through Kershaw. For its entire length, it is concurrent with SC 157 Truck. Its southern terminus is at the intersection of Marion Street and Minor Street which is also where SR 157 has its southern terminus and SR 341 passes through. As trucks are banned on East Marion Street through Kershaw, the truck route bypasses the downtown area. The routes head north along North Minor Street for three blocks to Hilton Street, US 601. The northbound directions of the two truck routes run along southbound US 601 forming a wrong-way concurrency. At North Hampton Street, the highway reaches US 521 Bus. and US 601 Bus. Continuing west along Hilton Street, they cross a railroad track before the truck routes terminate at US 521. US 601 from this point continues south along US 521 around the west side of the town.
