SmURFP


Small ultra red fluorescent protein is a class of far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein, α-allophycocyanin. Native α-allophycocyanin requires an exogenous protein, known as a lyase, to attach the chromophore, phycocyanobilin. Phycocyanobilin is not present in mammalian cells. smURFP was evolved to covalently attach phycocyanobilin without a lyase and fluoresce, covalently attach biliverdin and fluoresce, blue-shift fluorescence to match the organic fluorophore, Cy5, and not inhibit E. coli growth. smURFP was found after 12 rounds of random mutagenesis and manually screening 10,000,000 bacterial colonies.

Properties

smURFP is a homodimer with absorption and emission maximum of 642 nm and 670 nm, respectively. A tandem dimer smURFP was created and has similar properties to smURFP. smURFP is extremely stable with a protein degradation half-life of 17 hour and 33 hour without and with chromophore, respectively. This is comparable to the jellyfish-derived enhanced green fluorescent protein protein degradation half-life of 24 hour. smURFP is extremely photostable and out performs mCherry and tdTomato in living cells. The extinction coefficient of smURFP is extremely large and has a modest quantum yield, which makes it comparable biophysical brightness to eGFP and ~2-fold brighter than most red or far-red fluorescent proteins derived from coral. Despite being a homodimer, all tested N- and C- terminal fusions show correct cellular localization, including the difficult fusion to α-tubulin and Lamin B1. smURFP is named after the Smurfs, due to its light blue appearance in white light.
An Excel sheet of the smURFP absorbance, excitation, and emission spectra can be downloaded . The crystal structure of a smURFP mutant was published in .

smURFP used as nanoparticles, exogenous probes, and ''in vitro'' assays

Free smURFP is 2-3 nm in diameter. smURFP nanoparticles of ~10-14 nm diameter can be synthesized in an oil and water emulsion and remain fluorescent. These fluorescent protein nanoparticles are stable in living mice and useful for non-invasive tumor fluorescence imaging.
Free smURFP, purified protein and not genetically encoded, can be encapsulated into viruses and used for non-invasive, fluorescence imaging of biodistribution in living mice.
smURFP allows for the creation of in vitro assays to detect enzyme activity. An assay was developed for thrombin with a detection range of 1.07 aM–0.01 mM and a limit of detection of 0.2 aM.

Chromophore availability in cells and mice

Despite showing comparable biophysical brightness to eGFP when purified protein was normalized, this was not seen in living cells. This suggested there was not enough chromophore within cells. Addition of biliverdin increased fluorescence, but smURFP with biliverdin was not comparable to eGFP. Biliverdin has two carboxylates at neutral pH and this is inhibiting cellular entry. is a more hydrophobic analog and readily crosses the cellular membrane. smURFP with shows comparable fluorescence to eGFP in cells and is brighter than bacterial phytochrome fluorescent proteins.
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In mice, smURFP fluorescence is visible in HT1080 tumor xenografts without exogenous biliverdin, but fluorescence is less than coral-derived red fluorescent proteins, mCherry and mCardinal. Visible fluorescence is not always usable fluorescence and fluorescent proteins should always be compared to other useful, fluorescent proteins. Intravenous injection of exogenous biliverdin or does not increase fluorescence of smURFP expressed in tumors after 1 to 24 hours. Mass spectrometry showed that the ester groups were rapidly removed from . Addition of 25 μM biliverdin or dramatically increased fluorescence of excised tumors and smURFP is present without chromophore. Further research is necessary to optimize chromophore availability in mice to obtain fluorescence comparable or greater than coral-derived red fluorescent proteins.

Adding chromophore to cells

, , and are commercially available from . , biliverdin, or phycocyanobilin is dissolved in DMSO at a concentration of 5 mM. The solution is very dark and pipette vigorously to ensure all is dissolved. is not soluble in common buffers, including phosphate buffered saline or Hank's balanced salt solution. Add 1-5 μM in media or buffer containing 10% fetal bovine serum. Add 25 μM biliverdin to cells. Biliverdin does not saturate the smURFP sites and does not achieve maximum fluorescence intensity. should be used to get maximum fluorescence intensity. Incubate smURFP with chromophore for as long as possible to increase protein accumulation caused by enhanced protein stability with chromophore. Leave chromophore for a minimum of 3 hours and 24 h is recommended. Remove chromophore, wash with media containing 10% FBS, and image in media lacking phenol red or imaging buffer.

smURFP genetically encoded biosensors

Kinase FRET sensor. smURFP is a useful acceptor for many red fluorescent proteins due to spectral overlap. A rationally designed red fluorescent protein, stagRFP, allows for easier creation of FRET sensors. stagRFP is a useful FRET donor to the far-red acceptor smURFP and an ERK kinase FRET reporter was created with an average response of ~15%. The new sensor allowed for simultaneous visualization of three kinases, Src, Akt, ERK, in a single cell.
Fluorescently imaging the cell cycle. Pioneering work by Atsushi Miyawaki and coworkers developed the fluorescent ubiquitination-based cell cycle indicator, which enables fluorescence imaging of the cell cycle. Originally, a green fluorescent protein, mAG, was fused to hGem and an orange fluorescent protein was fused to hCdt1. Note, these fusions are fragments that contain a nuclear localization signal and ubiquitination sites for degradation, but are not functional proteins. The green fluorescent protein is made during the S, G2, or M phase and degraded during the G0 or G1 phase, while the orange fluorescent protein is made during the G0 or G1 phase and destroyed during the S, G2, or M phase. A far-red and near-infrared FUCCI was developed using a cyanobacteria-derived fluorescent protein and a bacteriophytochrome-derived fluorescent protein.