Semuliki National Park lies on Uganda's border with the Democratic Republic of the Congo. The Rwenzori Mountains are to the south-east of the park, while Lake Albert is to the park's north. The park lies within the Albertine Rift, the western arm of the East African Rift. The park is located on a flat to gently undulating landform that ranges from above sea level. The park experiences an average rainfall of, with peaks in rainfall from March to May and from September to December. Many areas of the park experience flooding during the wet season. The temperature at the park varies from, with relatively small daily variations. The park borders the Semliki and Lamia Rivers, which are watering places for many animals. The park has two hot springs in a hot mineral encrusted swamp. One of the springs - Mumbuga spring - resembles a geyser by forming a 0.5 m high fountain. These hot springs attract a large number of shorebirds and provide salt licks for many animals. From 1932 to 1993, the area covered by Semuliki National Park was managed as a forest reserve, initially by the colonial government and then by the Ugandan government's Forest Department. It was made a national park by the government in October 1993 to protect the forests as an integral part of the protected areas of the Western Rift Valley. The park is part of a network of protected areas in the Albertine Rift Valley. Other protected areas in this network include:
Park visitors can engage in birdwatching, walking to the savannah grassland, hiking through the Kirumia Trail, and visit the hot springs where the water is hot enough to cook eggs and plantain.
The forests in the park are of great socio-economic importance to the human communities that live near the park. The local people practise subsistence agriculture and use the park's forests to supplement their livelihoods. Some of the products they obtain from the forests include fruits and vegetables, herbal medicines, and construction materials. The local population is increasing at a rate of 3.4 percent per year. The high population density and declining agricultural productivity combined with an unavailability of alternative sources of income means that the local population is dependent on the park's resources. The forest also plays an important cultural and spiritual role in local people's lives. The forests are also the home of approximately 100 Basua people, an indigenous community who still largely live as hunter-gatherers. Because tourism provides the Basua people with an additional source of income, park visitors can learn more about the Basua people's culture and history at the park and see handmade crafts that they have produced. Past practises of the managing authorities that excluded the local people created resentment among them. This reduced the effectiveness of conservation practices and contributed to the occurrence of illegal activities. Since the 1990s, the Ugandan Wildlife Authority has involved the local communities in park planning. Civil unrest took place in the Bundibugyo District between 1997 and 2001. On 16 June 1997, Allied Democratic Forces rebels attacked and took over the town of Bundibugyo and occupied the park headquarters. The people who lived near the park were moved to internally displaced people's camps.