Sec61 or SecYEG is a membrane protein complex found in all domains of life. As the core component of the translocon, it transports proteins to the endoplasmic reticulum in eukaryotes and out of the cell in prokaryotes. It is a doughnut-shaped pore through the membrane with 3 different subunits, SecY, SecE, and SecG. It has a region called the plug that blocks transport into or out of the ER. This plug is displaced when the hydrophobic region of a nascent polypeptide interacts with another region of Sec61 called the seam, allowing translocation of the polypeptide into the ER lumen. Although SecY and SecE are conserved in all three domains of life, bacterial SecG is not homologous with eukaryotic Sec61β. The eukaryotic Sec61β is however homologous to the archaeal SecG, leading some authors to refer to the archaeal complex as SecYEβ instead of SecYEG.
Structure
Much of the knowledge on the structure of the SecY pore comes from an X-ray crystallography structure of its archaeal version. The large SecY subunit consists of two halves, trans-membrane segments 1-5 and trans-membrane segments 6-10. They are linked at the extracellular side by a loop between trans-membrane segments 5 and 6. SecY can open laterally at the front. SecE is a single spanning membrane protein in most species. It sits at the back of SecY, wrapping around the two halves of SecY. Secβ is not essential. Its sits on the side of SecY and makes only few contacts with it. In a side view, the channel has an hourglass shape, with a cytoplasmic funnel that is empty, and an extracellular funnel that is filled with a little helix, called the plug. In the middle of the membrane is a construction, formed from a pore ring of six hydrophobic amino acids that project their side chains inwards. During protein translocation, the plug is moved out of the way, and a polypeptide chain is moved from the cytoplasmic funnel, through the pore ring, the extracellular funnel, into the extracellular space. Hydrophobic segments of membrane proteins exit sideways through the lateral gate into the lipid phase and become membrane-spanning segments. The eubacterial SecY protein interacts with the signal sequences of secretory proteins as well as with two other components of the protein translocation system: SecA and SecE. SecY is an integral plasma membrane membrane protein of 419 to 492 amino acid residues that apparently contains 10 transmembrane, 6 cytoplasmic and 5 periplasmic regions.
Bakers yeast have two such homologous complexes; the essential one is named Sec61, and the non-essential one is called Ssh1. Like Sec61, Ssh1 does dock to the ribosome.
