He was the youngest son of John Morley, a hosiery manufacturer with premises in Nottingham who opened offices in Wood Street, London; his mother was Sarah Poulton of Maidenhead. Born in Homerton, From an early age he worked for his father's business in London. When his father and brothers chose to retire, he was left in managerial control. By 1860 he was sole owner of both the London and Nottingham parts of the business, and as it grew rapidly into the largest of its kind in the world he became very wealthy, and a model employer. Morley took a large residence in Stamford Hill, Stoke Newington when not living at his City of London address. He was a member of Thomas Binney's King's Weigh HouseCongregational Chapel in Fish Street Hill, London. He ventured into publishing, becoming one of the proprietors of the Daily News, the main Liberal paper of the period. By reducing its price, its losses turned to gains and carried greater influence. As a Liberal, he was one of Gladstone's keen supporters, and was elected as an MP for Nottingham in 1865, and later Bristol. In later life he became a strong advocate of temperance.
Philanthropist and educationalist
Sometimes called the "Philanthropic Merchant", he was sufficiently eminent to be caricatured in Vanity Fair. A contemporary biographer, writing in the Annual Register of World Events, considered that he would be remembered by posterity as "one of the leading merchant princes and philanthropists of the century". Among many philanthropic ventures, Morley endowed Morley College in London for adult education. He was also treasurer of Homerton College, chairing its inauguration on 20 April 1852 as the new "Training Institution of the Congregational Board of Education", following the purchase, extension and rebuilding on the site of the old mansion and buildings of Homerton Academy.
There is a statue to Samuel Morley in Bristol, and a second memorial above his place of interment in Dr Watts' Walk, Abney Park Cemetery, Stoke Newington, London. The latter is designed with elegant simplicity, as a large raised tomb, with a plain pediment to each end for nonconformists, even those as wealthy as Samuel Morley, generally resisted ostentatious memorials. A bust of Morley, by Joseph Else, is to be found at the Waverley Street entrance to The Arboretum, Nottingham with lettering beneath his likeness describing him as an MP, merchant and philanthropist. A Primitive Methodist chapel was erected in 1889 in Blue Bell Hill, Nottingham, and named in his memory the Morley Memorial Chapel.