Before being elected as a member of the Assembly of the Republic, Rubina Berardo worked as civil servant for the Regional Government of Madeira in the department European Affairs and External Cooperation since December 2005, a post she still holds.
Before being elected to the Assembly of the Republic, Rubina Berardo was an active member in the national and regional Social Democratic Youth, having run, unsuccessfully, for president of the latter in 2011. On October 2015, she was elected member of the Assembly of the Republic, for the constituency of Madeira, and assumed membership of the parliamentary committees for European Affairs and for Budget, Finances and Administrative Modernization. In representation of her constituency, Rubina Berardo has integrated the Commission of Inquiry to the process that led to the sale and settlement of Banco Internacional do Funchal. Four years later, on July 2019, the Madeiran newspaper, JM-Madeira, announced that Berardo was not running for re-election to the Assembly of the Republic. The decision for non-re-election to the Portuguese Parliament came from PSD-Madeira's president, Miguel Albuquerque, and the regional party's Political Commission as result from the negotiations made with PSD regarding the Closed lists.
Despite her stance on certain civil rights, Rubina has been a member of pro-life movement throughout her political career, having supported the movement "Sociedade Civil da Madeira Junta pela Vida" in Madeira which campaigned No in the Portuguese abortion referendum in 2007.
Euthanasia
Following the proposals submitted, In February 2020, by left-wing and liberal parties to the Assembly of the Republic on the legalization of euthanasia Berardo published an opinion article in JM-Madeira highly criticizing such proposals. In accordance with her Lutheran and pro-life background and beliefs, Berardo argued that the parties involved in these proposals are forcing their own agendas on the issue and failing the promises made of more civil society debates after these proposals failed parliamentary approval in 2018. She further questioned that parties' proposal regarding what is the precise moment when they consider that someone gives up their human dignity and thus must be subject to the process of euthanasia, because society, medicine and the state have failed such individual. In her view such moment does not exist since human dignity is inalienable.
Berardo advocates for Portugal's further integration in the European Union, while an increasing Madeira's political autonomy from the Portuguese central government. As a federalist she believes that social-economic development in Portugal is only achievable by giving regions more political power, instead of centralizing political power in Lisbon.
April 2017, Portuguese media denounced the fact the parliamentarians representing the Autonomous Regions were benefiting from a duplication of subsidies to cover their air fare costs. Parliamentarians representing Madeira and Azores were entitled not only to benefit from the subsidy available to all tax residents of the Autonomous Regions, as well as to benefit from the subsidy made available by the Portuguese Parliament to cover transportation costs. Unlike most of her colleagues, benefiting from the double subsidy, Rubina Berardo made clear to the press that she never applied for the subsidy made available to tax residents, since it was her understanding that the subsidy provided by the Portuguese parliament is more than enough to cover the air fare costs relating to the travels to her constituency. Out of the 12 Parliamentarians representing the Autonomous Region, Rubina Berardo, was the only one to opt to apply for one, of the two, available subsidies.