The R26 begins in the town of Rouxville, at a t-junction with the N6 National Route. It begins by going north-east for 30 kilometres to meet the western terminus of the R726 and bypass the town of Zastron. From Zastron, the R26 goes northwards for 65 kilometres, following the border with Lesotho, to the town of Wepener near the Van Rooyen's Gate border with Lesotho, where it meets the R702 Road. The R702 & R26 become one road for a few kilometres westwards. Just after crossing the Caledon River, at the junction with the R701 Road, the R702 remains on the road westwards while the R26 turns northwards. The R26 continues north-east, still following the Lesotho border, for 28 kilometres to the town of Hobhouse, where it meets the southern terminus of the R709 Road. From Hobhouse, the R26 goes north-east for 40 kilometres to reach a junction with the N8 National Route. They form one road eastwards for 12 kilometres before the R26 becomes its own road northwards and bypasses the town of Ladybrand to the east. From Ladybrand, the R26 continues northwards for 35 kilometres to the town of Clocolan, where it meets the southern terminus of the R708 Road. From Clocolan, the R26 goes eastwards for 30 kilometres to the town of Ficksburg, which is another border point into Lesotho. Just After Ficksburg, the R26 meets the southern terminus of the R70 Road. From the R70 junction, the R26 goes north-east for 40 kilometres, following the Lesotho borderline again, to the town of Fouriesburg, where it meets the western terminus of the R711 Road. From Fouriesburg, the R26 goes northwards for 48 kilometres to the city of Bethlehem. At the intersection with the N5 National Route in Bethlehem, as the straight road northwards is the R76 Road, the R26 joins the N5 eastwards for a few kilometres before becoming its own road northwards and meeting the western end of the R714 Road. From the R714 junction, the R26 goes northwards for 48 kilometres to bypass the town of Reitz and intersect with the R57 Road. From the R57 junction, the R26 proceeds northwards for 58 kilometres, bypassing Tweeling, to reach an intersection with the R34 Road. The R26 & R34 become one road eastwards, crossing the Wilge River into the town of Frankfort. East of Frankfort, the R26 becomes its own road, going north-north-east for 28 kilometres to the town of Villiers, where it ends at an off-ramp junction with the N3 National Route and the R103 Route just north of the Villiers Toll Plaza on the N3.
