Protocatechuic acid


Protocatechuic acid is a dihydroxybenzoic acid, a type of phenolic acid. It is a major metabolite of antioxidant polyphenols found in green tea. It has mixed effects on normal and cancer cells in in vitro and in vivo studies.

Biological effects

Protocatechuic acid is antioxidant and anti-inflammatory. PCA extracted from Hibiscus sabdariffa protected against chemically induced liver toxicity in vivo. In vitro testing documented antioxidant and anti-inflammatory activity of PCA, while liver protection in vivo was measured by chemical markers and histological assessment.
PCA has been reported to induce apoptosis of human leukemia cells, as well as malignant HSG1 cells taken from human oral cavities, but PCA was found to have mixed effects on TPA-induced mouse skin tumours. Depending on the amount of PCA and the time before application, PCA could reduce or enhance tumour growth. Similarly, PCA was reported to increase proliferation and inhibit apoptosis of neural stem cells. In an in vitro model using HL-60leukemia cells, protocatechuic acid showed an antigenotoxic effect and tumoricidal activity.
In two preclinical investigations, protocatechuic acid from Hibiscus sabdariffa L. showed an excellent ability to effectively inhibit the replication of herpes simplex virus type 2 and to potently inactivate the catalytic activity of urease

Occurrence in nature

Protocatechuic acid can be isolated from the stem bark of Boswellia dalzielii. and from leaves of Diospyros melanoxylon
The hardening of the protein component of insect cuticle has been shown to be due to the tanning action of an agent produced by oxidation of a phenolic substance. In the analogous hardening of the cockroach ootheca, the phenolic substance concerned is protocatechuic acid.

In foods

, obtained from the fruit of the açaí palm, is rich in protocatechuic acid., Protocatechuic acid also exists in the skins of some strains of onion as an antifungal mechanism, increasing endogenous resistance against smudge fungus. It is also found in Allium cepa.
PCA occurs in roselle, which is used worldwide as a food and beverage.
Protocatechuic acid is also found in mushrooms such as Agaricus bisporus or Phellinus linteus.

Metabolism

Protocatechuic acid is one of the main catechins metabolites found in humans after consumption of green tea infusions.

Enzymes

;Biosynthesis enzymes
; Degradation enzymes
The enzyme protocatechuate decarboxylase uses 3,4-dihydroxybenzoate to produce catechol and CO2.
The enzyme protocatechuate 3,4-dioxygenase uses 3,4-dihydroxybenzoate and O2 to produce 3-carboxy-cis,cis-muconate.