| Characters | Title | Character debut date | Notes | Country |
| Ryo Asuka | Devilman Crybaby | | Ryo Asuka is a genderless angel who is Akira Fudo's best friend, while Akira is the protagonist. Akira was the first person to show him kindness, causing him to become deeply attached. Near the end, Ryo remembers he is Satan, an intersex fallen angel who fell out of favor with God when he challenged God's authority for killing the demons and replacing them with humans. Satan set out to start a war between humans and demons and was reborn as a male. He fell in love with Akira and subconsciously had him turned into a devilman to ensure he would survive the war and be by his side. By the end of the series, Satan realizes he loves Akira despite having previously believed love did not exist and cries and mourns Akira's death. | Japan |
| Crimvael "Crim" | Interspecies Reviewers | January 11, 2020 | A well-endowed intersex angel with a broken halo, that has male and female genitalia, as noted throughout the series. Despite his feminine appearance, he chooses to identify as male upon meeting Stunk & Zel in the first episode, to avoid them trying anything perverted on him. He is hinted to be bisexual in Episode 3 and in the 9th episode the narrator uses the pronoun "they" to refer to Crim. | Japan |
| Luca Esposito | Astra Lost in Space | July 3, 2019 | Luca, in the anime and manga Astra Lost in Space, is an artist and talented engineer on board the Astra. He was raised as and mostly identifies as a boy, and comes out as intersex halfway through the story, in the episode "Secret," not considering himself a man or a woman. His characteristics imply he most likely has Klinefelter's or a milder type of Androgen Insensitivity Syndrome, and was described by Michele Liu of Anime News Network as "unique" since Luca is a main character which is born intersex rather than "altered by sci-fi space diseases or external influence," with Liu also describing Luca as bisexual. | Japan |
| Iena Madaraba | | February 18, 2020 | Iena Madaraba, also known as Yena, is a spotted hyena with male genitalia and is confused about her true gender & sexuality. Although she later finds out that she is biologically female, she still allows others to refer her with either gender pronoun in episodes such as "The Wild Habits of a Troubled Animal." | Japan |
| Ruby Moon | Cardcaptor Sakura | September 7, 1999 | Ruby Moon has no biological sex. They are always seen presenting as female, even though Spinel Sun often asks her why don't they present as male for a change as shown in episodes like "Sakura and the Mysterious Transfer Student." At some points, Ruby Moon states that their gender does not matter because they are not actually human and also that girls have cuter clothes. | - |
| Stevonnie | Steven Universe | January 15, 2015 | Stevonnie is a fusion of both Steven and Connie. Steven and Connie identify as male and female respectively, but the gender of Stevonnie is difficult to describe, with series creator Rebecca Sugar describing it as the "living relationship between Steven and Connie." Stevonnie is commonly referred to with gender neutral pronouns, while male and female characters seem to be physically attracted to Stevonnie. | United States |
| Izana Shinatose | Knights of Sidonia | | Izana belongs to a new, nonbinary third gender that originated during the hundreds of years of human emigration into space, as first shown in the episode "Commencement." Izana later turns into a girl after falling in love with Nagate Tanasake. | Japan |
| Characters | Name of comic | Years | Notes |
| Comet | Adventure Comics/Action Comics/Supergirl | 1989-1991 | Comet is an intersex character, who changed their form from female to male, and Comet later began relationship with Blithe, since she loved both their forms. |
| Desire | The Sandman | 1989-Present | Desire, one of the Endless, from Neil Gaiman The Sandman. |
| Rebis | Doom Patrol | 1963-1968 | Rebis is a character in the comic series Doom Patrol. |
| Richard Plantagenet, later Richard III of England | Requiem of the Rose King | 2013-Present | In this manga adaptation of Richard III, Richard is portrayed as intersex instead of hunchbacked as the character is normally depicted in the original play. |
| Ystina, the Shining Knight | Demon Knights | 2011-2013 | Ystina is revealed as DC Comics's first intersex character in Demon Knights #14. |
| Characters | Work | Author | Years | Description |
| Anjum | The Ministry of Utmost Happiness | Arundhati Roy | 2017 | A major character in this book who is intersex, Muslim and a hijra. |
| Cornelius Brunner | The Final Programme | Michael Moorcock | 1965 | Cornelius Brunner, the result of a physical merging of the protagonist Jerry Cornelius and Miss Brunner in this novel. |
| Therem Harth rem ir Estraven | The Left Hand of Darkness | Ursula K. Le Guin | 1969 | Therem is a character in this science fiction novel. |
| Fawn Singleton Farrell | No Matter How Much You Promise to Cook or Pay the Rent You Blew It Cauze Bill Bailey Ain't Never Coming Home Again | Edgardo Vega Yunqué | 2003 | Fawn Singleton Farrell is the half-sister of main character Vidamía Farrell, in this novel. |
| Swan Er Hong | 2312 | Kim Stanley Robinson | 2012 | Swan Er Hong is the lead character in this science fiction novel. |
| Ilario | Ilario, A Story of the First History | Mary Gentle | 2006-2007 | Ilario is the lead character in this set of alternate history novels. |
| Keral | The World Wreckers | Marion Zimmer Bradley | 1971 | Keral is a member of an alien species in this science fiction novel. |
| Kirsten Lattimer | None of the Above | I. W. Gregorio | 2015 | Kirsten, homecoming queen and champion hurdler, finds her life turned upside-down after a diagnosis with androgen insensitivity syndrome in this novel. |
| Callie Stephanides | Middlesex | Jeffrey Eugenides | 2002 | Callie "Cal" Stephanides is an intersex character in this novel. |
| Bel Thorne | Vorkosigan Saga | Lois McMaster Bujold | 1986-Present | Bel Thorne is an intersex ship captain in this series of science fiction novels. |
| Fitz Wahram | 2312 | Kim Stanley Robinson | 2013 | Fitz Wahram is an intersex character in this science fiction novel. |
| Max Walker | Golden Boy | Abigail Tarttelin | 2013 | Max is a lead character in this award-winning novel. |
| Annabel/Wayne | The Ministry of Utmost Happiness | Kathleen Winter | 2010 | Annabel/Wayne is an intersex character in this novel who was born intersex but is raised as a man. |
| Wraeththu | Wraeththu | Storm Constantine | 1987-1993 | Wraeththu is a member of a post-apocalyptic species in this set of novels. |
| Sadako Yamamura | Ring | Koji Suzuki | 1991-2013 | Sadako Yamamura is an antagonist in this novel series, later made into a film. |
| Characters | Actor | Show | Years | Notes |
| Amy | Jessica Campbell | Freaks and Geeks | 1999-2000 | Amy, girlfriend of Ken Miller in teen comedy/drama TV series, was said to be a groundbreaking intersex character on television. |
| Vincent Clarkson | Daphnée Duplaix, Phillip Jeanmarie, Siena Goines | Passions | 1999-2008 | An intersex character in this NBC/DirecTV soap opera who becomes pregant with his father's son. |
| Lauren Cooper | Bailey De Young | Faking It | 2014-2018 | Lauren is a character in MTV scripted comedy and said to be the first intersex series regular on television. |
| Rachel Green | Jennifer Aniston | Friends | 1994-2004 | Rachel, in this TV sitcom, was rumored to be intersex in episode The One with the Rumor. |
| Jake | Not Known | Emily Owens, M.D. | 2012-2013 | Jake, in the 2012 Emily Owens, M.D. episode "Emily and... the Question of Faith" that featured an intersex baby. |
| Peta | Leeanna Walsman | Janet King | 2014-2017 | Peta is character revealed as intersex in the final episode of the second Janet King series. |
| Raven | Amanda Saenz | Faking It | 2014-2018 | Raven was notable for being the first intersex character played by an intersex person. |
