| Villain | Media of origin | Members |
| The United Underworld | Batman: The Movie | The Joker, the Penguin, the Riddler and Catwoman. |
| The Red Triangle Circus Gang | Batman Returns | The Penguin, Organ Grinder, Poodle Lady, Tattooed Strongman, Acrobatic Thug, Terrifying Clown, Thin Clown, Fat Clown, Screaming Red Triangle Gang Thug, Rocket Launcher Clown, Sword Swallower, Devil Fire Breather, Knifethrower Dame, and Snake Lady |
| Brain Trust | Batman Beyond | Bombshell, the Invulnerable Man, and the Albino |
| The Chimeras | Batman Beyond | King Cobra, Ramhead, and the Tigress |
| The Jokerz | Batman Beyond | Various rosters |
| The Terrific Trio | Batman Beyond | Magma, Freon and the 2D Man |
| Team Penguin | The Batman | The Penguin, Killer Croc, the Rag Doll, Killer Moth, and the Firefly |
| The Joining | The Batman | Various unnamed extraterrestrials; alliance with Hugo Strange |
| The Fearsome Hand of Four | ' | Woman, Baton, Bulk, and Shot |
| Unnamed group led by Professor Pyg | Beware the Batman | Professor Pyg, Mister Toad, and the Man-Bat |
| Unnamed group hired by Ra's al Ghul | Beware the Batman | Professor Pyg, Mister Toad, Cypher, the Magpie, Tobias Whale, and Phosphorus Rex |
| The Animalitia | ' | The Penguin, Killer Croc, the Man-Bat, the Cheetah, and Silverback |
| The Maniax | Gotham | Jerome Valeska, Barbara Kean, Robert Greenwood, Aaron Helzinger, and Arnold Dobkins |
| Jerome's cult | Gotham | Jerome Valeska, Jeri, Dwight Pollard |
| The Monsters | Gotham | Fish Mooney and various Indian Hill monsters |
| Army of Freaks | Gotham | The Penguin, Fish Mooney, Poison Ivy, Mr. Freeze and Firefly |
| Legion of Horribles | Gotham | Jerome Valeska, the Penguin, Mr. Freeze, the Mad Hatter, Firefly, the Scarecrow and Solomon Grundy |
| The Children of Arkham | ' | , the Penguin, and the Blockbuster. |
| The Pact | ' | The Joker, Harley Quinn, the Riddler, Bane, Mr. Freeze, and Catwoman |
| Unnamed group | DC Super Heroes vs. Eagle Talon | The Joker, Harley Quinn, and the Penguin |
