Jurkat cells

History

Examples of derivatives

• The JCaM1.6 cell line is deficient in Lck activity due to the deletion of part of the LCK gene from the LCK transcript.
• J.RT3-T3.5 cells have a mutation in the T cell receptor beta chain locus precluding expression of this chain. This affects the cells in several ways; they do not express surface CD3 or produce the T cell receptor alpha/beta heterodimer. Since they are deficient in the TCR complex, these cells are a useful tool for transfection studies using T cell receptor alpha and beta chain genes and are widely used in labs in which T cell receptor gene transfer technologies are studied.
• The I 9.2 and I 2.1 cell lines. The I 2.1 cell line is functionally defective for FADD and the I 9.2 cell line is functionally defective for caspase-8, both defective molecules being essential to apoptosis or necroptosis of cells.
• The D1.1 cell line does not express the CD4 molecule, an important co-receptor in the activation pathway of helper T cells.
• The J.gamma1 subline contains no detectable phospholipase C-gamma1 protein and therefore has profound defects in T cell receptor calcium mobilization and activation of nuclear factor of activated T cells.
• J-Lat contains integrated but transcriptionally latent HIV proviruses, in which GFP replaces nef coding sequence, and a frameshift mutation in env.

  Cell line contamination