At Khirbet Sir, just east of Jayyus, two rock-cut tombs have been found, with a large mound with terraces cut in the sides, and a good well below. Byzantine ceramics have also been found.
Ottoman era
Jayyus was incorporated into the Ottoman Empire in 1517 with all of Palestine, and in 1596 it appeared in the tax registers as being in the Nahiya of Bani Sa'b of the Liwa of Nablus. It had a population of 24 households and 6 bachelors, all Muslim. The villagers paid taxes on wheat, barley, summer crops, olive trees, occasional revenues, goats and/or beehives; a total of 11,746 akçe. Half of the revenue went to a Muslim charitable endowment. In 1838, Robinson noted the village, called Jiyus, as being in the Beni Sa'ab district, west of Nablus. In 1882, the PEF's Survey of Western Palestine described Jiyus as a "moderate-sized stone village on a ridge, with olives to the south-east. It appears to be an ancient site, having rock-cut tombs and ancient wells." In the 19th century and early 20th century the village was dominated by the Palestinian el-Jayusah or Jayyusi clan.
British Mandate era
In the 1922 census of Palestine conducted by the British Mandate authorities, Jaiyus had a population of 433, all Muslims, increasing in the 1931 census to 569, again all Muslim, in a total of 147 houses. In the 1945 statistics the population of Jayyus consisted of 830 Muslims with a land area of 12,571 dunams according to an official land and population survey. Of this, 1,556 dunams were designated for plantations and irrigable land, 2,155 for cereals, while 22 dunams were built-up areas.
Since the Six-Day War in 1967, Jayyus has been under Israeli occupation. After the 1995 accords, 26.6% of Jayyus land is defined as Area B land, while the remaining 73.4% is defined as Area C land. The village is located in an area directly affected by the Israeli-built barrier and around 75 per cent of the village's farming land is on the other side of the wall. In order to get to their land the farmers need to use one of two gates, North gate or South gate, or gates number 943 and 979 respectively. The two gates are supposed to be open for three short periods a day. In June 2005, the gates were usually closed, and farmers were staging regular protests at the gates. As of February 2007, the gates are open for only three hours per day - one hour each morning, afternoon and evening on average. In 2002, Jayyus became the first village to mount a non-violent campaign with Israeli and international participation against the construction of the wall and the expansion of settlements on its land. According to The Financial Times, 50 percent of the once-prosperous Jayyus villagers are now dependent on foreign food aid because their agricultural land has been cut off by the wall. In January 2005 Ta'ayush activists along with Gush Shalom, the Israeli Committee Against House Demolitions, Machsom Watch and Anarchists Against the Wall, together with residents of the Palestinian village, began to plant hundreds of olive saplings which they had brought with them to the plot of land where the bulldozers of the settlers had uprooted hundreds of olive trees. Advocate Wiam Shbeyta, an activist of the Ta'ayush movement, said:
"In spite of the police and army assertions, we do not recognise the ownership of the settlers over this land. This land belongs to the Jayyus villagers and the company "Geulat HaKarka" which is associated with the settlers took control of it on the false assertion that it was sold to them. The matter is still awaiting legal review, and we will not allow the settlers to dictate facts on the ground, to grab Palestinian lands and to commence establishing a new settlement on it."