Biebl's best-known work is his Ave Maria, which sets portions of the Angelus as well as the Ave Maria. The piece was composed sometime before 1 May 1959. The original composition was in the key of D major, but changed to C major when it was published by Wildt’s Musikverlag in 1964. The piece was brought to the United States by the Cornell University Glee Club in 1970. The ensemble met Biebl while on tour in Germany, during a recording session at a radio network where Biebl was music director. Conductor Thomas A. Sokol was given a number of Biebl's works, premiering them after returning home. The Ave Maria quickly gained popularity, most notably after becoming part of the repertoire of Chanticleer. Although the Ave Maria was originally scored for male voices, in 1985 Biebl prepared additional arrangements for SAT/SATB and SAA/TTBB choirs. In 1998, Biebl prepared a fourth arrangement for SSA/SSAA choir. As part of the Hinshaw Music, Inc. sheet music catalog, the four versions have sold over 670,000 copies between 1992 and 2016.
Program note
Wilbur Skeels – who published some of Biebl's other works – prepared the following information about the piece for use in choral program notes. All or parts of the information in this note are commonly cited by choirs recording or performing the piece.
Instrumental arrangements
The San Francisco Renegades, an all-age Drum and Bugle Corps, first adapted sections of Biebl's Ave Maria in their 2003 show: "Red Skies At Night". In 2005 they played the piece as the opener to their show, "The Days of Future Past". In 2006 the Phantom Regiment Drum and Bugle Corps, an International World Class Corps based in Rockford, Illinois, used the piece in its 2006 field show "Faust," further expanding awareness of Biebl's arrangement. Both drum and bugle corps continue to perform Franz Biebl's Ave Maria as part of their yearly repertoire. Several transcriptions and arrangements of the Biebl "Ave Maria" have been written since 2010. One transcription is by Jerry Brubaker, horn player and arranger for 30 years with the US Navy Band, done after hearing the piece sung at a Navy funeral. It has been performed by the Navy Band horn section and the NIH Community Orchestra Horn Club on numerous occasions. In 2018 Triplo Press of Minneapolis, Minnesota, published an arrangement of the work by James Olcott for 12 trumpets. Pacific Crest Drum and Bugle Corps uses Franz Biebl's Ave Maria as their corps song.
In 2009–10, an arrangement of the Ave Maria for wind ensemble was the subject of litigation that reached the United States Supreme Court. At issue was whether a school district was justified in prohibiting an instrumental performance of the piece at a high school graduation ceremony due to its underlying religious nature. The United States Court of Appeals for the Ninth Circuit upheld the school district's actions. The Supreme Court declined to hear the case, but Justice Samuel Alito issued a rare written opinion dissenting from the Court's decision. In a footnote, Alito described Biebl's setting of the Ave Maria text as "relatively obscure" in comparison to settings by Franz Schubert, Charles Gounod, and other more well-known composers.