Fifi Forget-Me-Not is the cheerful hostess of the series. She says hello at the beginning of each episode and at the end she says goodbye and promises to come back soon, sometimes, anytime, another time, another day or maybe next time. When she forgets what to do, she says: "Fiddly Flowerpetals!" before describing the problem for the audience to guess. When she remembers, another character says: "Fifi Forget-Me-Not forgot!". Sometimes her catchphrase when she forgets what to do is "Buttercups and Daisies!", and when something is wrong she says: "Diggly Dandelions!" A sociable character, she likes to cook and look after plants. Fifi lives in a watering can house. Voiced by Jane Horrocks.
Aunt Tulip is Fifi's aunt. She likes to have tea parties and chats with the Flowertots. She lives in a pineapple house and has a pet grub named Grubby. He is very little, and often gets lost. Aunt Tulip is voiced by Tim Whitnall.
Buttercup is a buttercup. She lives in a "Milk Bucket House" with Daisy and has a mouse called Cornflower because of his lovely blue colour. Voiced by Joanna Ruiz.
Daisy is a daisy. She is a shy and dreamy Flowertot in comparison to her sister Buttercup. She is a little bit clumsy. She loves her comfort duckling toy called "Diddyduck".
Poppy is a poppy who is Fifi's friend. She has a market stall where she sells everything that the Flowertots need for each episode. Lives in a pumpkin house.
Primrose is Fifi's friend. She is prim and doesn't like getting messy. She always wants to look magnificent. She gets upset when something goes wrong for her. She likes to create apparel for herself and tries to dress Fifi up. Her catchphrase is "I am NOT having a good day!" Voiced by Jane Horrocks.
Violet is Fifi's friend. She is very creative and loves painting. Violet lives with Primrose in a marrow house and is voiced by Maria Darling.
Pip Gooseberry is the youngest of the Flowertots. He likes to help Fifi and Fuzzbuzz in the garden. Sometimes he is too noisy and gets too excited. Fifi looks after him when Stingo and Slugsy play tricks on him. He lives in the gooseberry bushes on his playground and is voiced by Maria Darling.
Bumble is a bumblebee and Fifi's best friend. He often visits her and is always ready to come to her rescue but isn't good at landing and usually crashes. His catchphrase is "Wiggly Worms!" and he is voiced by Marc Silk.
Stingo is a wasp who causes trouble for the Flowertots. He tries to steal food such as cake, blueberries and chocolate. Lives in a red apple house. His catchphrase is "Rotten Raspberries!" and he is voiced by Tim Whitnall.
Hornetto is Stingo's cousin, a guitar playing hornet who is even nastier than Stingo on occasion.
Slugsy is a slug and Stingo's sidekick. He is always ready to help Stingo but is very slow and often accidentally ruins Stingo's plans. He loves Primrose but doesn't want to admit it. He lives at the top of Stingo's house. He speaks with a lisp and is voiced by Marc Silk.
Flutterby is a butterfly, a friendly character. He takes Buttercup and Daisy around the garden and has a landing pad near the girls' house.
Webby is a spider. She is the eldest and the wisest in Flowertot Garden. The Flowertots ask her for advice and help, but Stingo and Slugsy avoid her so as not to hit her web. So-called because she lives in a web house, she is voiced by Maria Darling.
Mo is Fifi's lawnmower, which takes the harvest and the Flowertots around the garden.
Diggly is a grumpy but funny worm. He lives on the compost heap and eats scraps but prefers pumpkin pies, He is voiced by John Thomson and Marc Silk
