Djoehana was born in what was the Dutch East Indies. His father, Raden Rangga Wiradikarta, was wedana of Majalaya south of Bandung, and belonged to the Sundanesenobility, as his mother was. Djoehana attended STOVIA and graduated in 1918. He was then appointed as a district physician in Bawean Island. After that he held various positions in the colonial government health system, in various cities in Sumatra and Java. In 1930, he was sent to Amsterdam University in the Netherlands, where he got his "European" Doctor of Medicine degree the following year. Back in the Netherlands Indies, he was the director of the public hospital of Ambarawa in Central Java when Japanese troops landed in 1942. During the Japanese occupation of Indonesia, Djoehana was deputy director of the laboratory of the Eijkman Institute in Jakarta. On 17 September 1945, exactly one month after the proclamation of Indonesian Independence, Djoehana was appointed to a committee of five members formed by President Sukarno to prepare the establishment of the Indonesian Red Cross. In 1947, Djoehana was appointed gewoon hoogleraar at what was to become the faculty of medicine of the University of Indonesia in 1950. From 1951 to 1954, he was head of the Landskoepokinrichting, which later became Pasteur Institute of Indonesia, then state-owned pharmaceutical companyPT Bio Farma. He was the first native Indonesian to head this organization, having been in existence since colonial times. In 1953, he was appointed "professor extraordinary" in microbiology and serology at the Faculty of Sciences the University of Indonesia in Bandung, established in 1950. In 1956, Djoehana was appointed vice-president of the foundation in charge of setting up a faculty of medicine at Padjadjaran University, of which he became the first dean. In 1957, he became the first native Indonesian to hold the position of dean of the Faculty of Sciences of the University of Indonesia in Bandung, replacing Dutch Professor H. Th. M. Leeman. This faculty was the last in Indonesia to still be headed by a Dutchman, because of difficulties in finding a qualified person required in the initial years of independence. As dean of the Faculty of Sciences, Djoehana participated in the establishment of the Bandung Institute of Technology in 1959 and was appointed a member of the Presidium of the ITB on March 2, 1959 until November 1, 1959 when Prof. Ir. R. O. Kosasih was appointed as definitive Rector of ITB. Djoehana was married to the sister of Sjahrir, the first prime minister of the Republic of Indonesia from 1945 to 1947, and founder of the Indonesian Socialist Party
