Their introduction saw the withdrawal of the last of Glasgow's venerable Class 303 "Blue Train" sets which had been in service since 1960. Although deliveries started to Strathclyde in 1999, the 334s did not enter passenger service until 2001 due to numerous teething problems and technical failures which plagued the new units at their introduction. SPT had ordered 38 Juniper units. The technical problems led to an extra two Class 170 and 2 additional Class 334 units being added as a goodwill gesture. ScotRail leased a total of 40 units which can be seen in use daily across the SPT electric network. On the maiden run of the units, 334010 and 334020 ran to Gourock. Initially the units ran Ayrshire Coast Line and Inverclyde Line but then become a common sight on the Argyle Line and North Clyde Line. In December 2010, the Class 334s operated the AyrshireCoast Line, Inverclyde Line, North Clyde Line and Argyle Line. By June 2011 the class was substantially replaced by the Class 380 units on the Ayrshire and Inverclyde lines, with only occasional units seen on those routes.
Relivery and Refurbishment
In September 2008, the Scottish Government's agency Transport Scotland announced that all ScotRail trains would be eventually repainted in a new, blue livery with white Saltire markings on the carriage ends. Class 334 re-livery began in November 2010 in preparation for the Airdrie to Bathgate reopening. On 24 August 2011, ScotRail announced plans to refurbish all the fleet, with the project expected to start in November 2011 and take two years to complete. 334020 was the first to be properly refurbished at Kilmarnock's Brodie Rail Works. It was there for 3 months from 3 February 2012 to 2 May 2012. The last unit to receive a full refurbishment was 334006 which left Brodie Works on 8 November 2014. From early 2015, an extensive overhaul has been carried out by Alstom to the entire fleet to improve passenger conditions, which included the retrofitting of full air conditioning, at-seat charging points, provision for wi-fi, and reliability improvements to the doors. Additionally, a new CCTV system for Driver Only Operation was installed.
Accidents
On 16 January 2008, 334017 was involved in a minor collision whilst entering Glasgow Central station. The unit was working the 08:24 Gourock to Glasgow Central service when it collided with an empty, stationary Class 318 unit. The train was travelling at less than when the collision occurred. Four passengers were slightly injured; however only one required hospital attention, and was transferred to Glasgow Southern General hospital in a taxi. The unit was undamaged.
On 15 October 2015 at 17:05, 334016 was involved in a collision on the approach to Uphall railway station. The unit was working the 16:39 Edinburgh to Milngavie service when it collided with an empty red Toyota Hilux pick-up truck which was stolen and abandoned on the tracks. The train was travelling at approximately when the collision occurred. One passenger suffered minor injuries to their hand from broken glass. The unit did not derail.