| Rank | Heat | Nation | Athletes | Time | Notes |
| 1 | 2 | | Aleksandr Brednev, Konstantin Petriashov, Roman Smirnov, Aleksandr Khyutte | 39.37 | Q |
| 2 | 3 | | Yip Siukeung, Lai Chunho, Leung Kiho, Ho Manloklawrence | 39.53 | Q |
| 3 | 1 | | Hannes Dreyer, Simon Magakwe, Rapula Sefanyetso, Thuso Mpuang | 39.56 | Q |
| 4 | 2 | | Yang Yang, Huan Minhua, Kao Haichao, Zheng Dongsheng | 39.57 | Q |
| 5 | 3 | | Weerawat Pharueang, Wachara Sondee, Sompote Suwannarangsri, Suppachai Chimdee | 39.81 | Q |
| 6 | 3 | | Martynas Jurgilas, Rytis Sakalauskas, Martas Skrabulis, Aivaras Pranckevičius | 40.05 | q |
| 7 | 2 | | Kang Li Loong Calvin, Muhammad Amirudin Jamal, Lee Cheng Wei, Yeo Fu Ee Gary | 40.33 | q, SB |
| 8 | 3 | | Richard Davenport, James Alaka, Rion Pierre, Ashley Bryant | 40.38 | |
| 9 | 2 | | Emmanuel Bamise, Obinna Metu, Stanley Gbagbeke, Ogho-Oghene Egwero | 40.43 | |
| 10 | 3 | | Kelvin Asare, Enoch Sekum, Elorm Amenakpor, Michael Tinadu | 41.13 | |
| 11 | 1 | | Goabaone Moitoi, Thapelo Ketlogetswe, Tirafalo Batsholelwang, Keene Motukisi | 41.19 | Q |
| 12 | 3 | | Mohd Zabidi Ghazali, Mohammad Noor Imran A Hadi, Latif Nyat, Nor Mohd Ikhwan | 41.50 | |
| 13 | 3 | | Gregor Areseb, Johannes Maritz, Jesse Uri Khob, Lee Roy Bock | 41.51 | |
| 14 | 1 | | Abdullah Said Alabri, Mohammed Khalfan Al Mayahi, Ouf Hamed Aloufi, Ismail Alkindi | 43.76 | |
| 1 | | Peter Matthews, Jason Young, Rasheed Dwyer, Jacques Harvey | | 170.14 |
| 2 | | Ryota Yamagata, Yusuke Kotani, Sota Kawatsura, Shōta Iizuka | | 170.14 |
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