Activation-induced cell death


AICD is programmed cell death caused by the interaction of Fas receptors and Fas ligands. AICD is a negative regulator of activated T lymphocytes that results from repeated stimulation of their T-cell receptors and helps to maintain peripheral immune tolerance. Alteration of the process may lead to autoimmune diseases.
The AICD effector cell is one that expresses FasL, and apoptosis is induced in the cell expressing the Fas receptor. Both activated T cells and B cells express Fas and undergo clonal deletion by the AICD mechanism. Activated T cells that express both Fas and FasL may be killed by themselves or by each other.

Signaling

The binding of Fas ligand to Fas receptor triggers trimerization of Fas, whose cytoplasmic domain is then able to bind the death domain of the adaptor protein FADD. Procaspase 8 binds to FADD's death effector domain and proteolytically self-activates as caspase 8. Fas, FADD, and procaspase 8 together form a death-inducing signaling complex. Activated caspase 8 is released into the cytosol, where it activates the caspase cascade that initiates apoptosis.

Regulation of Fas-FasL and AICD

FasL is primarily regulated at the transcriptional level. NFAT activated by TCR stimulation activates FasL transcription, possibly indirectly by upregulating early growth response proteins. T cell activation-induced transcription of FasL is further regulated by c-Myc–MAX heterodimers, and can be blocked by c-Myc downregulation. Interferon regulatory factors IRF1 and IRF2 also upregulate FasL transcription by directly binding to the FasL promoter.
Not much is known about the regulation of Fas and other death receptors. However, overexpression of the protein CFLAR inhibits Fas-mediated apoptosis.