| Rank | Heat | Nation | Athletes | Time | Notes |
| 1 | 1 | | Chijindu Ujah, Harry Aikines-Aryeetey, Adam Gemili, Nethaneel Mitchell-Blake | 38.11 | Q, WL, *WC |
| 2 | 1 | | Rodrigo do Nascimento, Jorge Vides, Derick Silva, Paulo André de Oliveira | 38.22 | Q, SB, *WC |
| 3 | 2 | | Eseosa Desalu, Lamont Marcell Jacobs, Davide Manenti, Filippo Tortu | 38.29 | Q, SB, *WC |
| 4 | 3 | | Mike Rodgers, Justin Gatlin, Isiah Young, Cameron Burrell | 38.34 | Q, SB, *WC |
| 5 | 2 | | Wu Zhiqiang, Xie Zhenye, Su Bingtian, Liang Jinsheng | 38.46 | Q, SB, *WC |
| 6 | 2 | | Emre Zafer Barnes, Jak Ali Harvey, Izzet Safer, Ramil Guliyev | 38.47 | q, SB, *WC |
| 7 | 3 | | Mouhamadou Fall, Jimmy Vicaut, Mickaël-Meba Zézé, Stuart Dutamby | 38.51 | Q, SB, *WC |
| 8 | 1 | | Nesta Carter, Julian Forte, Rasheed Dwyer, Tyquendo Tracey | 38.51 | Q, SB, *WC |
| 9 | 1 | | Emile Erasmus, Simon Magakwe, Anaso Jobodwana, Akani Simbine | 38.66 | SB, *WC |
| 10 | 2 | | Christopher Garia, Churandy Martina, Hensley Paulina, Taymir Burnet | 38.67 | SB, *WC |
| 11 | 3 | | Gavin Smellie, Aaron Brown, Brendon Rodney, Andre De Grasse | 38.76 | SB |
| 12 | 2 | | Zdeněk Stromšík, Jan Veleba, Jan Jirka, Dominik Záleský | 38.77 | NR |
| 13 | 2 | | Ruttanapon Sowan, Nutthapong Veeravongratanas, Jirapong Meenapra, Siripol Punpa | 38.82 | |
| 14 | 2 | | Oleksandr Sokolov, Emil Ibrahimov, Volodymyr Suprun, Serhiy Smelyk | 38.84 | SB |
| 15 | 3 | | Wei Tai-sheng, Wang Wei-hsu, Yang Chun-han, Cheng Po-yu | 38.89 | SB |
| 16 | 1 | | Kevin Kranz, Patrick Domogala, Julian Reus, Robin Erewa | 38.91 | SB |
| 17 | 1 | | Trae Williams, Alex Hartmann, Zach Holdsworth, Jake Doran | 39.05 | |
| 18 | 3 | | Christopher Valdez, Yohandris Andújar, Mayovanex de Óleo, Yancarlos Martínez | 39.12 | |
| 19 | 1 | | Mochammad Bisma Diwa Abina, Lalu Muhammad Zohri, Eko Rimbawan, Bayu Kertanegara | 39.39 | SB |
| 20 | 3 | | Otto Ahlfors, Ville Myllymäki, Oskari Lehtonen, Samuel Purola | 39.59 | SB |
| 21 | 1 | | Alberto Aguilar, Rafael Vázquez, Alexis Nieves, Abdel Kalil | 39.76 | SB |
| 1 | | Remigiusz Olszewski, Przemysław Słowikowski, Karol Kwiatkowski, Dominik Kopeć | | |
| 2 | | Dicksson Kamungeremu, Tatenda Tsumba, Itayi Vambe, Ngoni Makusha | | R163.3 |
| 3 | | Shuhei Tada, Ryota Yamagata, Yuki Koike, Yoshihide Kiryu | | 170.6 |
| 3 | | Enoch Adegoke, Emmanuel Arowolo, Ogho-Oghene Egwero, Usheoritse Itsekiri | | 170.6 |
| Rank | Lane | Nation | Athletes | Time | Notes | Points |
| 7 | | Rodrigo do Nascimento, Jorge Vides, Derick Silva, Paulo André de Oliveira | 38.05 | WL | 8 |
| 5 | | Mike Rodgers, Justin Gatlin, Isiah Young, Noah Lyles | 38.07 | SB | 7 |
| 4 | | Chijindu Ujah, Harry Aikines-Aryeetey, Adam Gemili, Nethaneel Mitchell-Blake | 38.15 | | 6 |
| 4 | 8 | | Wu Zhiqiang, Xie Zhenye, Su Bingtian, Liang Jinsheng | 38.16 | SB | 5 |
| 5 | 9 | | Stuart Dutamby, Jimmy Vicaut, Mickaël-Meba Zézé, Mouhamadou Fall | 38.31 | SB | 4 |
| 6 | 3 | | Nesta Carter, Julian Forte, Rasheed Dwyer, Nigel Ellis | 38.88 | | 3 |
| 7 | 2 | | Emre Zafer Barnes, Jak Ali Harvey, Izzet Safer, Ramil Guliyev | 39.13 | | 2 |
| 6 | | Eseosa Desalu, Lamont Marcell Jacobs, Davide Manenti, Filippo Tortu | | | 0 |
London, Great Britain
Nassau, Bahamas
Gainesville, United States