2016 Atlantic Sun Men's Basketball Tournament
The 2016 Atlantic Sun Men's Basketball Tournament marked the 37th year the league conducted a postseason tournament. The tournament was held March 1–6, 2016 at campus sites as top seeds hosted each round. Florida Gulf Coast won their second Atlantic Sun Tournament title and a berth in the 2016 NCAA Tournament.
Seeds
All 8 teams in the conference participated in the Tournament.Stetson was ineligible to participate in the NCAA Tournament due to APR violations, but was allowed to participate in the Atlantic Sun Tournament. Had Stetson won the tournament, North Florida would have gone to the NCAA Tournament because of its regular season Atlantic Sun Conference title.
Teams were seeded by record within the conference, with a tiebreaker system to seed teams with identical conference records.
| Seed | School | Conference | Tiebreaker |
| 1 | North Florida | 10–4 | |
| 2 | NJIT | 8–6 | 2–0 vs. Jacksonville, 1–1 vs. FGCU |
| 3 | Jacksonville | 8–6 | 0–2 vs. NJIT, 2–0 vs. FGCU |
| 4 | Florida Gulf Coast | 8–6 | 1–1 vs. NJIT, 0–2 vs. Jacksonville |
| 5 | Kennesaw State | 7–7 | 2–0 vs. Lipscomb |
| 6 | Lipscomb | 7–7 | 0–2 vs. Kennesaw State |
| 7 | Stetson | 4–10 | 1–1 vs. USC Upstate, 1–1 vs. North Florida |
| 8 | USC Upstate | 4–10 | 1–1 vs. Stetson, 0–2 vs. North Florida |