| Rank | Heat | Lane | Nation | Athletes | Time | Notes |
| 1 | 2 | 7 | | Nesta Carter, Kemar Bailey-Cole, Julian Forte, Andrew Fisher | 37.71 | Q, CR |
| 2 | 1 | 5 | | Richard Kilty, Harry Aikines-Aryeetey, James Ellington, Daniel Talbot | 37.93 | Q, SB |
| 3 | 1 | 8 | | Keston Bledman, Marc Burns, Rondel Sorrillo, Richard Thompson | 38.09 | Q, SB |
| 4 | 2 | 5 | | Bruno de Barros, Jefferson Lucindo, Aldemir da Silva Junior, Jorge Vides | 38.10 | Q, SB |
| 5 | 2 | 4 | | Arnaud Remy, Jimmy Vicaut, Ben Bassaw, Christophe Lemaitre | 38.33 | q, SB |
| 6 | 1 | 3 | | Kazuma Oseto, Kei Takase, Yoshihide Kiryu, Shōta Iizuka | 38.34 | q, SB |
| 7 | 1 | 2 | | Yaniel Carrero, Roberto Skyers, Reynier Mena, Yadier Cuéllar | 38.44 | SB |
| 8 | 1 | 6 | | Giovanni Codrington, Churandy Martina, Jorén Tromp, Hensley Paulina | 38.52 | SB |
| 9 | 2 | 6 | | Remigiusz Olszewski, Dariusz Kuć, Kamil Masztak, Karol Zalewski | 38.60 | SB |
| 10 | 3 | 4 | | Aleixo-Platini Menga, Lucas Jakubczyk, Julian Reus, Martin Keller | 38.62 | Q, SB |
| 11 | 3 | 8 | | Gavin Smellie, Dontae Richards-Kwok, Jared Connaughton, Justyn Warner | 38.70 | Q, SB |
| 12 | 3 | 7 | | Jason Rogers, Antoine Adams, Lestrod Roland, Brijesh Lawrence | 38.76 | SB |
| 13 | 3 | 3 | | Emil Ibragimov, Serhiy Smelyk, Ihor Bodrov, Vitaliy Korzh | 38.91 | SB |
| 14 | 2 | 8 | | Chen Shiwei, Xie Zhenye, Yang Yang, Mo Youxue | 39.00 | SB |
| 15 | 1 | 7 | | Jin Su Jung, Jarrod Geddes, Jake Hammond, Alexander Hartmann | 39.21 | SB |
| 16 | 2 | 2 | | Andrew Hinds, Levi Cadogan, Ackeem Forde, Ramon Gittens | 39.27 | SB |
| 17 | 2 | 3 | | Dubeiker Cedeño, Arturo Ramírez, Álvaro Luis Cassiani, Diego Rivas | 39.29 | SB |
| 18 | 1 | 4 | | Kemar Hyman, Tyrell Cuffy, David Hamil, Rhymiech Adolphus | 39.76 | SB |
| 3 | 2 | | Marvin Bracy, Trell Kimmons, Rakieem Salaam, Charles Silmon | | R170.7 |
| 3 | 5 | | Jonathan Farquharson, Adrian Griffith, Stephen Newbold, Blake Bartlett | | R170.7 |
| 3 | 6 | | | | |
| Rank | Lane | Nation | Athletes | Time | Notes |
| 9 | 7 | | Emil Ibrahimov, Serhiy Smelyk, Ihor Bodrov, Vitaliy Korzh | 38.53 | =NR |
| 10 | 6 | | Yaniel Carrero, Roberto Skyers, Reynier Mena, Yadier Cuéllar | 38.60 | |
| 11 | 8 | | Chen Shiwei, Xie Zhenye, Yang Yang, Mo Youxue | 38.83 | SB |
| 12 | 3 | | Allistar Clarke, Jason Rogers, Antoine Adams, Brijesh Lawrence | 39.07 | |
| 13 | 4 | | Remigiusz Olszewski, Dariusz Kuć, Jakub Adamski, Karol Zalewski | 39.31 | |
| 1 | | Jin Su Jung, Jarrod Geddes, Jake Hammond, Alexander Hartmann | | |
| 5 | | Giovanni Codrington, Churandy Martina, Jorén Tromp, Hensley Paulina | | |
| 2 | | | | |
| Rank | Lane | Nation | Athletes | Time | Notes | Points |
| 6 | | Nesta Carter, Nickel Ashmeade, Julian Forte, Yohan Blake | 37.77 | | 8 |
| 4 | | Keston Bledman, Marc Burns, Rondel Sorrillo, Richard Thompson | 38.04 | SB | 7 |
| 5 | | Richard Kilty, Harry Aikines-Aryeetey, James Ellington, Dwain Chambers | 38.19 | | 6 |
| 4 | 8 | | Bruno de Barros, Jefferson Lucindo, Aldemir da Silva Junior, Jorge Vides | 38.40 | | 5 |
| 5 | 2 | | Kazuma Oseto, Kei Takase, Yoshihide Kiryu, Shōta Iizuka | 38.40 | | 4 |
| 6 | 7 | | Gavin Smellie, Dontae Richards-Kwok, Jared Connaughton, Justyn Warner | 38.55 | SB | 3 |
| 7 | 3 | | Aleixo-Platini Menga, Lucas Jakubczyk, Julian Reus, Martin Keller | 38.69 | | 2 |
| 1 | | | | | |
