| Rank | Heat | Lane | Nation | Athletes | Time | Notes |
| 1 | 1 | 1 | | Nigel Levine, Michael Bingham, Rabah Yousif, Martyn Rooney | 3:00.65 | Q |
| 2 | 1 | 5 | | Mame-Ibra Anne, Teddy Venel, Mamoudou Hanne, Thomas Jordier | 3:00.80 | Q, SB |
| 3 | 1 | 3 | | Thomas Schneider, Miguel Rigau, David Gollnow, Jonas Plass | 3:02.41 | Q, SB |
| DQ | 2 | 4 | | Nikita Uglov, Pavel Ivashko, Pavel Trenikhin, Vladimir Krasnov | 3:03.19 | Q |
| 5 | 2 | 5 | | Michał Pietrzak, Kacper Kozłowski, Andrzej Jaros, Rafał Omelko | 3:03.52 | Q, SB |
| 6 | 2 | 2 | | Brian Gregan, Brian Murphy, Richard Morrissey, Thomas Barr | 3:03.57 | Q, NR |
| 7 | 2 | 7 | | Julien Watrin, Antoine Gillet, Michaël Bultheel, Kevin Borlée | 3:03.83 | q |
| 8 | 1 | 4 | | Jan Tesař, Daniel Němeček, Michal Desenský, Patrik Šorm | 3:04.07 | q, SB |
| 9 | 2 | 8 | | Pau Fradera, Samuel García, Lucas Bua, Mark Ujakpor | 3:04.68 | SB |
| 10 | 1 | 2 | | Davide Re, Michele Tricca, Lorenzo Valentini, Matteo Galvan | 3:04.74 | SB |
| 11 | 1 | 7 | | Bjorn Blauwhof, Terrence Agard, Obed Martis, Liemarvin Bonevacia | 3:05.93 | |
| 12 | 2 | 3 | | Batuhan Altıntaş, Halit Kiliç, İlham Tanui Özbilen, Yavuz Can | 3:07.68 | |
| 13 | 1 | 8 | | Festus Asante, Andreas Bube, Nick Ekelund-Arenander, Nicklas Hyde | 3:08.12 | |
| 14 | 2 | 6 | | Silvan Lutz, Daniele Angelella, Philipp Weissenberger, Johannes Wagner | 3:08.63 | SB |
| 15 | 2 | 1 | | Staša Vrhovec, Mateo Kovačić, Yann Eloi Senjarić, Mateo Ružić | 3:12.73 | |
| 1 | 6 | | Vitaliy Butrym, Yevhen Hutsol, Danylo Danylenko, Volodymyr Burakov | | R 163.2 |
