| Rank | Heat | Lane | Name | Nationality | Time | Note |
| 1 | 2 | 5 | Adam Gemili | | 10.37 | Q |
| 2 | 5 | 2 | Tyreek Hill | | 10.37 | Q |
| 3 | 7 | 7 | Aaron Ernest | | 10.39 | Q |
| 4 | 4 | 4 | Carlos Nascimento | | 10.43 | Q, PB |
| 5 | 8 | 9 | Jazeel Murphy | | 10.45 | Q |
| 6 | 4 | 9 | Hassan Taftian | | 10.48 | Q |
| 7 | 7 | 9 | Chijindu Ujah | | 10.48 | Q |
| 8 | 3 | 3 | Zharnel Hughes | | 10.50 | Q |
| 9 | 9 | 5 | Odean Skeen | | 10.50 | Q |
| 10 | 6 | 4 | Kazuma Oseto | | 10.50 | Q |
| 11 | 7 | 8 | Cejhae Greene | | 10.52 | q |
| 12 | 8 | 5 | Patrick Domogala | | 10.56 | Q |
| 13 | 6 | 9 | Hugh Donovan | | 10.57 | Q |
| 14 | 9 | 9 | Kazuki Kanamori | | 10.57 | Q, PB |
| 15 | 1 | 3 | Teray Smith | | 10.58 | Q |
| 16 | 3 | 2 | Yousef Ali Al-Shalani | | 10.59 | Q |
| 17 | 9 | 2 | Fana Mofokeng | | 10.59 | q |
| 18 | 1 | 9 | Giovanni Galbieri | | 10.60 | Q |
| 19 | 1 | 7 | Siphelo Ngquboza | | 10.60 | Q |
| 20 | 6 | 2 | Devon Rittinger | | 10.62 | q |
| 21 | 9 | 4 | Eetu Rantala | | 10.64 | q |
| 22 | 8 | 8 | Ben Jaworski | | 10.65 | q |
| 23 | 1 | 5 | Titus Kafunda | | 10.67 | |
| 24 | 2 | 4 | Zhenye Xie | | 10.68 | Q |
| 25 | 5 | 3 | Mobolade Ajomale | | 10.68 | Q |
| 26 | 4 | 5 | Robert Polkowski | | 10.68 | |
| 27 | 4 | 8 | Abdelghani Zghali | | 10.68 | |
| 28 | 2 | 2 | Mike Mokamba Nyang'Au | | 10.70 | |
| 29 | 9 | 6 | Héctor Ruiz | | 10.70 | |
| 30 | 5 | 9 | Tahir Walsh | | 10.71 | |
| 31 | 5 | 5 | Pierre Chalus | | 10.72 | |
| 32 | 6 | 3 | Jonathan Holder | | 10.73 | |
| 33 | 8 | 7 | Denis Ogarkov | | 10.73 | |
| 34 | 2 | 9 | Khalfani Muhammad | | 10.76 | |
| 35 | 1 | 4 | Rodrigo Rocha | | 10.78 | |
| 36 | 7 | 6 | Yuri Monteiro | | 10.78 | |
| 37 | 9 | 8 | Marek Bakalár | | 10.78 | |
| 38 | 8 | 6 | Anthony Farrington | | 10.78 | |
| 39 | 3 | 4 | Artur Bruno Rojas | | 10.80 | |
| 40 | 6 | 6 | J'maal Alexander | | 10.80 | NJR |
| 41 | 6 | 5 | Hensley Paulina | | 10.81 | |
| 42 | 7 | 5 | Aleksandr Eliseev | | 10.83 | |
| 43 | 4 | 3 | Ashron Sobers | | 10.84 | |
| 44 | 8 | 3 | Dumisani Bhebhe | | 10.84 | |
| 45 | 5 | 7 | Mateo Edward | | 10.86 | |
| 46 | 7 | 3 | Georgy Gusto | | 10.87 | |
| 47 | 3 | 7 | Andy Martínez | | 10.88 | |
| 48 | 1 | 2 | Eid Abdulla Al-Kuwari | | 10.95 | |
| 49 | 6 | 8 | César Ramírez | | 10.97 | |
| 50 | 2 | 8 | Kamil Bijowski | | 10.99 | |
| 51 | 2 | 1 | Kodi Harman | | 10.99 | |
| 52 | 3 | 9 | Volodymyr Suprun | | 11.00 | |
| 53 | 1 | 6 | Ali Omar Abdulla Al Doseri | | 11.02 | SB |
| 54 | 5 | 8 | Zdenek Stromšík | | 11.02 | |
| 55 | 7 | 1 | Fiacre Bahorou | | 11.05 | |
| 56 | 3 | 5 | Yanis Dallay | | 11.07 | PB |
| 57 | 9 | 7 | Davron Atabaev | | 11.11 | |
| 58 | 5 | 4 | Adrián Pérez | | 11.18 | |
| 59 | 6 | 7 | Yang Zi Xian | | 11.24 | |
| 60 | 2 | 6 | A. Kumara Makkawitaliyanage | | 11.32 | |
| 61 | 7 | 4 | Siueni Filimone | | 11.41 | PB |
| 62 | 9 | 3 | Samuel Olo Manel | | 11.46 | PB |
| 63 | 4 | 6 | Md Ariful Islam | | 11.48 | PB |
| 64 | 3 | 6 | Archel Evrard Biniakounou | | 11.49 | PB |
| 65 | 5 | 6 | Muhammad Sajid | | 11.76 | PB |
| 66 | 7 | 2 | Nicholas Chianese | | 11.76 | PB |
| 67 | 2 | 3 | George Manebona | | 11.78 | PB |
| 68 | 8 | 2 | Elama Fa’atonu | | 11.90 | PB |
| 69 | 2 | 7 | Sidney Quaresma | | 11.96 | PB |
| 70 | 1 | 8 | Andre Lopes de Pina | | 12.02 | PB |
| 71 | 8 | 4 | Daniel Philimon | | 13.31 | SB |
| – | 3 | 8 | Tamunotonye Briggs | | – | DNS |
| – | 4 | 2 | Okeudo Jonathan Nmaju | | – | DNS |
| – | 4 | 7 | Oumar Diallo | | – | DNS |
